STUDY AN COMPARISON OF DIAGNOSTIC METHODS (CULTURE – MOLECULAR) FOR BRUCELLA IN THE SAMPLES ISOLATED FROM PATIENTS SUSPECTED TO BRUCELLOSIS IN DIFFERENT REGION TEHRAN

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TALEBZADEH SARA; ZAKER BOSTANABAD SAEED; NAZARI RAZIEH

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Paper Information

Journal: NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL Year:2016 | Volume:6 | Issue:23 Page(s): 105-109

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Title

STUDY AN COMPARISON OF DIAGNOSTIC METHODS (CULTURE – MOLECULAR) FOR BRUCELLA IN THE SAMPLES ISOLATED FROM PATIENTS SUSPECTED TO BRUCELLOSIS IN DIFFERENT REGION TEHRAN

Author(s)

TALEBZADEH SARA | ZAKER BOSTANABAD SAEED | NAZARI RAZIEH | Issue Writer Certificate

Keywords

BRUCELLOSISQ2

CULTUREQ2

PCRQ2

COMPARISONQ2

Abstract

Aim and Background: BRUCELLOSIS is a major cause of zoonosis and in many countries, including Iran, is a public health concern. Developed a new diagnostic method with high sensitivity and minimize the risk of infection in laboratory personnel, is of paramount importance. Including the ways in which these benefits are polymerase chain reaction (PCR). In this study diagnostic methods (culture - molecular) Brucella isolated in samples from patients suspected of having BRUCELLOSIS in various parts of Tehran are compared and evaluated. Materials and Methods: 100 patients suspected of having symptoms of BRUCELLOSIS to the laboratory Masoud Tehran, the Bagheat Allah Hospital, Medical Sciences Iran went for blood sampling. Each blood sample medium (Castaneda) inoculation and temperature conditions 370c and %5 carbon dioxide was 7 to 30 days. Brucella strains standard of laboratory reference preparation and were stored under standard conditions. All articles published in the Brucella specific primers collect and studied were specific primers B4/B5 223 bp to reproduction a piece of the gene.Results: Two Brucella strains were isolated from 100 blood samples of the patients with BRUCELLOSIS by blood CULTURE and DNA isolated by Kit Cinnagen and after the PCR, fifteen were positive. The sensitivity of PCR, %100 specificity and respectively %86, 73.Conclusion: consideration great importance of molecular diagnosis of infectious diseases even at 10 nanograms of bacterial DNA in laboratory samples, also the high sensitivity of the method used in the diagnosis of such diseases on the basis of the results of this study and previous research PCR technique compared with CULTURE method appears to be that the sensitivity is higher. So this could be the reason for the replacement of this method as a diagnostic method in the laboratory.

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APA: Copy

TALEBZADEH, SARA, ZAKER BOSTANABAD, SAEED, & NAZARI, RAZIEH. (2016). STUDY AN COMPARISON OF DIAGNOSTIC METHODS (CULTURE – MOLECULAR) FOR BRUCELLA IN THE SAMPLES ISOLATED FROM PATIENTS SUSPECTED TO BRUCELLOSIS IN DIFFERENT REGION TEHRAN. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, 6(23), 105-109. SID. https://sid.ir/paper/204724/en

Vancouver: Copy

TALEBZADEH SARA, ZAKER BOSTANABAD SAEED, NAZARI RAZIEH. STUDY AN COMPARISON OF DIAGNOSTIC METHODS (CULTURE – MOLECULAR) FOR BRUCELLA IN THE SAMPLES ISOLATED FROM PATIENTS SUSPECTED TO BRUCELLOSIS IN DIFFERENT REGION TEHRAN. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL[Internet]. 2016;6(23):105-109. Available from: https://sid.ir/paper/204724/en

IEEE: Copy

SARA TALEBZADEH, SAEED ZAKER BOSTANABAD, and RAZIEH NAZARI, “STUDY AN COMPARISON OF DIAGNOSTIC METHODS (CULTURE – MOLECULAR) FOR BRUCELLA IN THE SAMPLES ISOLATED FROM PATIENTS SUSPECTED TO BRUCELLOSIS IN DIFFERENT REGION TEHRAN,” NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, vol. 6, no. 23, pp. 105–109, 2016, [Online]. Available: https://sid.ir/paper/204724/en

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