STUDY OF CK19 TUMOR MARKER EXPRESSION IN THE LUMINAL A BREAST CANCER CELL LINES

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SOLEIMANI REIHANEH; OLOOMI MANA; MAHDIAN REZA; KEYVANI GHAMSARI SAEIDEH; KARIMI NASRIN

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Journal: NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL Year:2016 | Volume:6 | Issue:23 Page(s): 20-23

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Title

STUDY OF CK19 TUMOR MARKER EXPRESSION IN THE LUMINAL A BREAST CANCER CELL LINES

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Keywords

BREAST CANCERQ1

CYTOKERATINQ1

CELL LINEQ2

LUMINAL AQ1

Abstract

Aim and Background: CYTOKERATINs (CKs) constitute the largest intermediate filamentous protein that during the development of malignancy, the original CK profile of the cell is frequently variable. CYTOKERATIN 19 (CK19) is known as an epithelial cell marker and CK19 expression was seen in more than 90% of BREAST CANCERs. In this study, CK19 mRNA expression by the real-time PCR (Polymerase Chain Reaction) in LUMINAL A CELL LINEs was assessed. Materials and Methods: MCF7 and T47D BREAST CANCER CELL LINEs were purchased and cultured according to the recommended protocols. The cells were collected, counted and RNA was extracted from CELL LINEs. The mRNA of CK19 as the target gene and GAPDH (Glyceraldehyde 3-phosphate Dehydrogenase) as a reference gene by real-time PCR was amplified. Quantitative CK19 expression was calculated using the DDCt method. The average ΔCt of CK19 and GAPDH was calculated in each CELL LINE as well as the DCt of GAPDH as a calibrator in different CELL LINEs was quantitatively reported.Results: This method can be used for detection of CK19 tumor marker expression, in the cells. On the other hand, the same expression in the MCF7 and T47D BREAST CANCER CELL LINEs was shown. Conclusions: Expression of CK19 is the same, in the LUMINAL A BREAST CANCER CELL LINEs. Molecular markers could be used for CELL LINEs classification that it is significant in BREAST CANCER research and it is helpful in detection and treatment of the disease.

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APA: Copy

SOLEIMANI, REIHANEH, OLOOMI, MANA, MAHDIAN, REZA, KEYVANI GHAMSARI, SAEIDEH, & KARIMI, NASRIN. (2016). STUDY OF CK₁₉ TUMOR MARKER EXPRESSION IN THE LUMINAL A BREAST CANCER CELL LINES. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, 6(23), 20-23. SID. https://sid.ir/paper/204727/en

Vancouver: Copy

SOLEIMANI REIHANEH, OLOOMI MANA, MAHDIAN REZA, KEYVANI GHAMSARI SAEIDEH, KARIMI NASRIN. STUDY OF CK₁₉ TUMOR MARKER EXPRESSION IN THE LUMINAL A BREAST CANCER CELL LINES. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL[Internet]. 2016;6(23):20-23. Available from: https://sid.ir/paper/204727/en

IEEE: Copy

REIHANEH SOLEIMANI, MANA OLOOMI, REZA MAHDIAN, SAEIDEH KEYVANI GHAMSARI, and NASRIN KARIMI, “STUDY OF CK₁₉ TUMOR MARKER EXPRESSION IN THE LUMINAL A BREAST CANCER CELL LINES,” NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, vol. 6, no. 23, pp. 20–23, 2016, [Online]. Available: https://sid.ir/paper/204727/en

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