OPTIMIZATION OF BOVINE SERUM ALBUMIN FIBRILLATION BY CONGORED SPECTROPHOTOMETRIC METHOD FOR USING AS NANOBIOMATERIAL

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ARASTEH AMIR; SALEHZADEH ALI; HABIBI REZAEI MEHRAN; MOOSAVI MOVAHEDI ALI AKBAR

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Journal: NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL Year:2016 | Volume:6 | Issue:23 Page(s): 65-75

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Title

OPTIMIZATION OF BOVINE SERUM ALBUMIN FIBRILLATION BY CONGORED SPECTROPHOTOMETRIC METHOD FOR USING AS NANOBIOMATERIAL

Author(s)

ARASTEH AMIR | SALEHZADEH ALI | HABIBI REZAEI MEHRAN | MOOSAVI MOVAHEDI ALI AKBAR | Issue Writer Certificate

Keywords

BOVINE SERUM ALBUMINQ1

AMYLOIDQ1

SPECTROPHOTOMETRYQ2

CONGOREDQ1

Abstract

Aim and Background: AMYLOIDs are fibrilar protein structures produced from aggragation of proteins and peptide together. In recent years AMYLOID fibrils are being noticed as new experimental nanomaterials and from this view the induction of proteins to AMYLOIDs could be beneficial. In this study, the fibrillation of BOVINE SERUM ALBUMIN (BSA) as a model protein is optimized. Materials and Methods: Different pH of 1, 2, 3, 4.7 and 7.4 of BOVINE SERUM ALBUMIN was prepared in Mixed Citrate–Phosphate buffer and agitated for 0, 24, 48 and 72 hour in 100 RPM in various temperature (40–80oC) for preparing protein fibrils. Then the amount of AMYLOID fibrils was detected by spectrophotometric CONGORED binding assay.Results: In this research, effect of four variables include temperature, pH, time of incubation and protein concentration was investigated on fibrillogenesis and results were confirmed by CONGORED spectrophotometric method as lmax and absorbance in lmax (Almax) accompanying with transmission electron microscopy. The optimum condition for fibrilogenesis was specified in 5 mg.ml-1 of protein and buffer pH of 3 after 72 hour incubation in 70oC.Conclusion: Simple CONGORED spectrophotometric method could be used as primary test for evaluating protein nanobiofibrils and absorbance in lmax is introduced as a valid indicator in this way.

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APA: Copy

ARASTEH, AMIR, SALEHZADEH, ALI, HABIBI REZAEI, MEHRAN, & MOOSAVI MOVAHEDI, ALI AKBAR. (2016). OPTIMIZATION OF BOVINE SERUM ALBUMIN FIBRILLATION BY CONGORED SPECTROPHOTOMETRIC METHOD FOR USING AS NANOBIOMATERIAL. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, 6(23), 65-75. SID. https://sid.ir/paper/204732/en

Vancouver: Copy

ARASTEH AMIR, SALEHZADEH ALI, HABIBI REZAEI MEHRAN, MOOSAVI MOVAHEDI ALI AKBAR. OPTIMIZATION OF BOVINE SERUM ALBUMIN FIBRILLATION BY CONGORED SPECTROPHOTOMETRIC METHOD FOR USING AS NANOBIOMATERIAL. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL[Internet]. 2016;6(23):65-75. Available from: https://sid.ir/paper/204732/en

IEEE: Copy

AMIR ARASTEH, ALI SALEHZADEH, MEHRAN HABIBI REZAEI, and ALI AKBAR MOOSAVI MOVAHEDI, “OPTIMIZATION OF BOVINE SERUM ALBUMIN FIBRILLATION BY CONGORED SPECTROPHOTOMETRIC METHOD FOR USING AS NANOBIOMATERIAL,” NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, vol. 6, no. 23, pp. 65–75, 2016, [Online]. Available: https://sid.ir/paper/204732/en

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