Phylogenetic analysis of COXI region in some Iranian goat breeds

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Seyedsharifi Reza; Badbarin Nejat

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Journal: JOURNAL OF ANIMAL ENVIRONMENT Year:2019 | Volume:11 | Issue:1 Page(s): 119-124

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Title

Phylogenetic analysis of COXI region in some Iranian goat breeds

Author(s)

Seyedsharifi Reza | Badbarin Nejat | Issue Writer Certificate

Keywords

Cytochrome Oxidase I geneQ1

genetic diversityQ2

phylogenetic analysisQ1

Iranian goatQ1

Abstract

Mitochondrial DNA has been one of the most widely used molecular markers for phylogenetic studies in animals because of its simple genomic structure. This study examines the genetic characteristic of domestic goat using sequence analysis of mitochondrial DNACytochrome oxidase subunit I (COI) to identify and differentiate among three common breeds (Adani, Najdi and Markhoz) of Iran. The genomic DNA was isolated by salting out method and amplified Cytochrome Oxidase I gene using PCR method with a pair of primer. Phylogenetic trees and pairwise calculations were obtained by using Mega 6 software. The COXI sequence from 60 individuals with haplotype diversity was 0. 47 and a nucleotide diversity was 0. 0500. The analyzes with the BioEdit Composition procedure indicated that the sequence for COXI was 28. 87% nucleotide A, 25. 25% nucleotide C, 15. 86% nucleotide G and 29. 66% nucleotide T, which had a C + G ratio of 42% and A + T is 58%. phylogenetic analysis of haplotype in the combination with the goat from Gen Bank showed that Iranian goat clustered in a separate lineage. This study was found informative for establishing relationships between breeds from different parts of the world. This study may facilitate the future researchers and breeders for better understanding the genetic interactions and breed differentiation for devising future breeding and conservation strategies to preserve the rich animal genetic reservoir of the country.

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APA: Copy

Seyedsharifi, Reza, & Badbarin, Nejat. (2019). Phylogenetic analysis of COXI region in some Iranian goat breeds. JOURNAL OF ANIMAL ENVIRONMENT, 11(1 ), 119-124. SID. https://sid.ir/paper/206469/en

Vancouver: Copy

Seyedsharifi Reza, Badbarin Nejat. Phylogenetic analysis of COXI region in some Iranian goat breeds. JOURNAL OF ANIMAL ENVIRONMENT[Internet]. 2019;11(1 ):119-124. Available from: https://sid.ir/paper/206469/en

IEEE: Copy

Reza Seyedsharifi, and Nejat Badbarin, “Phylogenetic analysis of COXI region in some Iranian goat breeds,” JOURNAL OF ANIMAL ENVIRONMENT, vol. 11, no. 1 , pp. 119–124, 2019, [Online]. Available: https://sid.ir/paper/206469/en

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