EFFECT OF VARIOUS FACTORS ON EXTRACELLULAR ALKALINE METALLOPROTEASE PRODUCTION OF A NEWLY ISOLATED MODERATELY HALOPHILE, SALINIVIBRIO SP. STRAIN AF- 2004

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AMOUZGAR M.A.; FATEMI A.Z.; KARBALAEI HEYDARI H.R.

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Journal: JOURNAL OF SCIENCE (UNIVERSITY OF TEHRAN) (JSUT) Year:2007 | Volume:32 | Issue:4 (SECTION: BIOLOGY 2) Page(s): 317-326

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Title

EFFECT OF VARIOUS FACTORS ON EXTRACELLULAR ALKALINE METALLOPROTEASE PRODUCTION OF A NEWLY ISOLATED MODERATELY HALOPHILE, SALINIVIBRIO SP. STRAIN AF- 2004

Author(s)

AMOUZGAR M.A. | FATEMI A.Z. | KARBALAEI HEYDARI H.R. | Issue Writer Certificate

Keywords

SALINIVIBRIOQ3

ALKALINE PROTEASEQ3

HALOALKA-LOPHILEQ3

MODERATELY HALOPHILIC BACTERIAQ3

PROTEASE PRODUCTIONQ3

Abstract

Production of extracellular protease was demonstrated under stress conditions of high temperature and high salinity by newly isolated moderate halophile, SALINIVIBRIO sp. strain AF-2004 in basal medium containing peptone, beef extract, Glucose, and NaCl. Modified Kunitz method was used for protease assay. The isolate was capable of producing protease in the presence of sodium chloride, sodim sulfate, sodium nitrate, sodium nitrite, potassium chloride, sodium acetate and sodium citrate. The maximum PROTEASE PRODUCTION was secreted in the presence 7.5 to 10% (w/v) sodium sulfate or 3% (w/v) sodium acetate (4.6 U ml-1). Various carbon sources induced enzyme production. The pH, temperature and agitation optima for enzyme production were 9.0, 32 DC and 220 rpm, respectively. Maximum protease activity was exhibited in the medium containing 1% (w/v) NaCl. Its optimum temperature was about 60°C and the maximum activity obtained when reaction time was 5 minutes. Maximum activity of the enzyme was at pH 8-9 and its activity was inhibited by EDTA. This result suggests that strain AF-2004 produces an extra-cellular alkaline metaloprotease moderately thermo-tolerant and stable at high salt concentrations.

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APA: Copy

AMOUZGAR, M.A., FATEMI, A.Z., & KARBALAEI HEYDARI, H.R.. (2007). EFFECT OF VARIOUS FACTORS ON EXTRACELLULAR ALKALINE METALLOPROTEASE PRODUCTION OF A NEWLY ISOLATED MODERATELY HALOPHILE, SALINIVIBRIO SP. STRAIN AF- 2004. JOURNAL OF SCIENCE (UNIVERSITY OF TEHRAN) (JSUT), 32(4 (SECTION: BIOLOGY 2)), 317-326. SID. https://sid.ir/paper/2077/en

Vancouver: Copy

AMOUZGAR M.A., FATEMI A.Z., KARBALAEI HEYDARI H.R.. EFFECT OF VARIOUS FACTORS ON EXTRACELLULAR ALKALINE METALLOPROTEASE PRODUCTION OF A NEWLY ISOLATED MODERATELY HALOPHILE, SALINIVIBRIO SP. STRAIN AF- 2004. JOURNAL OF SCIENCE (UNIVERSITY OF TEHRAN) (JSUT)[Internet]. 2007;32(4 (SECTION: BIOLOGY 2)):317-326. Available from: https://sid.ir/paper/2077/en

IEEE: Copy

M.A. AMOUZGAR, A.Z. FATEMI, and H.R. KARBALAEI HEYDARI, “EFFECT OF VARIOUS FACTORS ON EXTRACELLULAR ALKALINE METALLOPROTEASE PRODUCTION OF A NEWLY ISOLATED MODERATELY HALOPHILE, SALINIVIBRIO SP. STRAIN AF- 2004,” JOURNAL OF SCIENCE (UNIVERSITY OF TEHRAN) (JSUT), vol. 32, no. 4 (SECTION: BIOLOGY 2), pp. 317–326, 2007, [Online]. Available: https://sid.ir/paper/2077/en

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