APPLICATION OF KLEBSIELLA PNEUMONIAE IN DETERMINATION OF PULLULAN POLYMER AND OPTIMIZATION OF ACTIVITY FACTORS

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GHEZELBASH GH.R.; NAHVI I.; EMTIAZI G.; TAVASOLI M.

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Journal: IRANIAN JOURNAL OF BIOLOGY Year:2007 | Volume:19 | Issue:4 Page(s): 458-463

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Title

APPLICATION OF KLEBSIELLA PNEUMONIAE IN DETERMINATION OF PULLULAN POLYMER AND OPTIMIZATION OF ACTIVITY FACTORS

Author(s)

GHEZELBASH GH.R. | NAHVI I. | EMTIAZI G. | TAVASOLI M. | Issue Writer Certificate

Keywords

ULLULANQ3

PULLULANASEQ3

K.PNEUMONIAE PTCC 1053Q3

Abstract

PULLULANse is an enzyme that can be Produced by K.Pneumoniae, Bacillus acidopullulyticus and e few other Bacteria. This Enzme can brak pULLULAN biopolymer specifically and it is the most reliable method in this polymer detection. A suppernatant of K.Penamonia culture those cotains pULLULANase used in detection and measurment of pULLULAN. At first, K.Penamonia was cultured in a preliminaty active nutrient agar containing pULLULAN (the enzyme specific substrate ) for 24 hours .Then 2ml of this culture was added to the 16ml of a culture media and it was incubated in 370 C and 120 rpm.The Enzymatic activity of this strain was determined at 5 hour intervals and the activity was seen after 10 hours the enzymatic activity of produced pULLULANase measured according to released maltotrise in gram/lit, and 32.43g/l of maltotriose were release after 10 hours. The PH=5 and 400 C are the optimimum condition for enzymes activity.

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APA: Copy

GHEZELBASH, GH.R., NAHVI, I., EMTIAZI, G., & TAVASOLI, M.. (2007). APPLICATION OF KLEBSIELLA PNEUMONIAE IN DETERMINATION OF PULLULAN POLYMER AND OPTIMIZATION OF ACTIVITY FACTORS. IRANIAN JOURNAL OF BIOLOGY, 19(4), 458-463. SID. https://sid.ir/paper/21033/en

Vancouver: Copy

GHEZELBASH GH.R., NAHVI I., EMTIAZI G., TAVASOLI M.. APPLICATION OF KLEBSIELLA PNEUMONIAE IN DETERMINATION OF PULLULAN POLYMER AND OPTIMIZATION OF ACTIVITY FACTORS. IRANIAN JOURNAL OF BIOLOGY[Internet]. 2007;19(4):458-463. Available from: https://sid.ir/paper/21033/en

IEEE: Copy

GH.R. GHEZELBASH, I. NAHVI, G. EMTIAZI, and M. TAVASOLI, “APPLICATION OF KLEBSIELLA PNEUMONIAE IN DETERMINATION OF PULLULAN POLYMER AND OPTIMIZATION OF ACTIVITY FACTORS,” IRANIAN JOURNAL OF BIOLOGY, vol. 19, no. 4, pp. 458–463, 2007, [Online]. Available: https://sid.ir/paper/21033/en

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