DETECTION OF KLEBSIELLA PNEUMONIAE 16S RDNA SPECIFIC GENE BY PCR-ELISA TECHNIQUE

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

TAYEBEH F.; AMANI J.; MORADYAR M.; MIRHOSSAINI SA.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF ADVANCED BIOMEDICAL SCIENCES Year:2016 | Volume:5 | Issue:4 Page(s): 542-550

Download Full-Text

Persian Verion

View:

2,455

Download:

Cites:

Information Journal Paper

Title

DETECTION OF KLEBSIELLA PNEUMONIAE 16S RDNA SPECIFIC GENE BY PCR-ELISA TECHNIQUE

Author(s)

TAYEBEH F. | AMANI J. | MORADYAR M. | MIRHOSSAINI SA. | Issue Writer Certificate

Keywords

ENTEROBACTERIACEAEQ2

KLEBSIELLA PNEUMONIAEQ1

16S RDNAQ2

PCR-ELISAQ1

Abstract

Objective: KLEBSIELLA PNEUMONIAE is one of the most important infection bacteria of the ENTEROBACTERIACEAE family. It is also the second factor of hospital bacteremia due to the gram negative bacteria after Escherichia coli. In this research, this bacterium with the private 16SrDNA gene, was identified through PCR-ELISA technique.Materials and Methods: In this method the dNTP labeled with Digoxigenin was used for amplifying the specific gene. Streptavidin was loaded in ELISA plate, and then the specific Biotinylated probe was used to connect the Polymerase Chain Reaction (PCR) product. Biotinylated probe was connected to the streptavidin and the amplified gene was attached to the probe. Finally, the digoxigenin antibodies were used to identify the PCR product. The reaction was measured with an ELISA reader.Result: 16S RDNA of KLEBSIELLA PNEUMONIAE was amplified using the gene specific primers resulted in a fragment of the bp 260. The results of PCR-ELISA showed that this technique does not cross-react with the bacteria in their families. Additionally, the sensitivity of 6.0 ng was evaluated.Conclusion: In this research, PCR-ELISA technique was known as an accurate and rapid test for the detection of infection agents by using the specific gene. PCR-ELISA can act as an alternative method instead of time-consuming, less sensitive, and expensive techniques such as culture bacteria in blood agar plates, Macconkey agar, Realtime PCR and differential biochemical tests which are currently used in the research labs.

Cites

NOVEL PCR-ELISA TECHNIQUE AS A GOOD SUBSTITUTE IN MOLECULAR ASSAY

References

No record.

Cite

APA: Copy

TAYEBEH, F., AMANI, J., MORADYAR, M., & MIRHOSSAINI, SA.. (2016). DETECTION OF KLEBSIELLA PNEUMONIAE 16S RDNA SPECIFIC GENE BY PCR-ELISA TECHNIQUE. JOURNAL OF ADVANCED BIOMEDICAL SCIENCES, 5(4), 542-550. SID. https://sid.ir/paper/203863/en

Vancouver: Copy

TAYEBEH F., AMANI J., MORADYAR M., MIRHOSSAINI SA.. DETECTION OF KLEBSIELLA PNEUMONIAE 16S RDNA SPECIFIC GENE BY PCR-ELISA TECHNIQUE. JOURNAL OF ADVANCED BIOMEDICAL SCIENCES[Internet]. 2016;5(4):542-550. Available from: https://sid.ir/paper/203863/en

IEEE: Copy

F. TAYEBEH, J. AMANI, M. MORADYAR, and SA. MIRHOSSAINI, “DETECTION OF KLEBSIELLA PNEUMONIAE 16S RDNA SPECIFIC GENE BY PCR-ELISA TECHNIQUE,” JOURNAL OF ADVANCED BIOMEDICAL SCIENCES, vol. 5, no. 4, pp. 542–550, 2016, [Online]. Available: https://sid.ir/paper/203863/en

Related Journal Papers