COMPARISON OF METHICILLIN RESISTANCE PHENOTYPE WITH MECA GENE CARRIAGE IN CLINICAL ISOLATES OF STAPHYLOCOCCUS EPIDERMIDIS

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RAEI F.; EFTEKHAR F.

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Journal: IRANIAN JOURNAL OF BIOLOGY Year:2010 | Volume:23 | Issue:1 Page(s): 108-114

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Title

COMPARISON OF METHICILLIN RESISTANCE PHENOTYPE WITH MECA GENE CARRIAGE IN CLINICAL ISOLATES OF STAPHYLOCOCCUS EPIDERMIDIS

Author(s)

RAEI F. | EFTEKHAR F. | Issue Writer Certificate

Keywords

MECAQ3

STAPHYLOCOCCUS EPIDERMIDISQ2

PCRQ1

METHICILLINQ2

Abstract

Coagulase negative, METHICILLIN resistant Staphylococci specifically STAPHYLOCOCCUS EPIDERMIDIS are important causes of nosocomial infections. In addition, most of these isolates show multi-resistance to a number of other antibiotics including b-lactams. In this study, METHICILLIN resistance phenotype was compared to the presence of METHICILLIN resistance gene (MECA gene) among the isolates. Fifty five clinical isolates collected from 3 hospitals in Tehran were tested for antibiotic susceptibility using disc diffusion and minimal inhibitory concentrations (MIC) were measured for meticillin using the broth microdilution assay. Presence of the MECA gene in the clinical isolates was detected using specific primers and PCR. The disk susceptibility results showed that 50isolates (90.9 %) including 8 strains with intermediate inhibition zones were resistant to METHICILLIN. MIC results showed 78.1 % METHICILLIN resistance with MIC values ³4 mg/ml. MIC results for the intermediately resistant isolates revealed 5 sensitive and 3 resistant strains. PCR results showed that all METHICILLIN resistant isolates determined by disc diffusion and MIC assays carried the MECA gene except one isolate. Surprisingly, 4 isolates with METHICILLIN sensitive phenotype contained the MECA gene as well. The results of this study suggest that presence of the MECA gene does not necessarily account for METHICILLIN resistance. Ultimately, gene expression which is controlled by the environment, would determine the outcome.

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References

COMPARISON OF OXACILLIN AGAR SCREENING AND PCR METHODS IN DETECTION OF METHICILLIN RESISTANCE STAPHYLOCOCCUS EPIDERMIDIS STRAINS ISOLATED FROM BLOOD CULTURES OF CHILDREN

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APA: Copy

RAEI, F., & EFTEKHAR, F.. (2010). COMPARISON OF METHICILLIN RESISTANCE PHENOTYPE WITH MECA GENE CARRIAGE IN CLINICAL ISOLATES OF STAPHYLOCOCCUS EPIDERMIDIS. IRANIAN JOURNAL OF BIOLOGY, 23(1), 108-114. SID. https://sid.ir/paper/21051/en

Vancouver: Copy

RAEI F., EFTEKHAR F.. COMPARISON OF METHICILLIN RESISTANCE PHENOTYPE WITH MECA GENE CARRIAGE IN CLINICAL ISOLATES OF STAPHYLOCOCCUS EPIDERMIDIS. IRANIAN JOURNAL OF BIOLOGY[Internet]. 2010;23(1):108-114. Available from: https://sid.ir/paper/21051/en

IEEE: Copy

F. RAEI, and F. EFTEKHAR, “COMPARISON OF METHICILLIN RESISTANCE PHENOTYPE WITH MECA GENE CARRIAGE IN CLINICAL ISOLATES OF STAPHYLOCOCCUS EPIDERMIDIS,” IRANIAN JOURNAL OF BIOLOGY, vol. 23, no. 1, pp. 108–114, 2010, [Online]. Available: https://sid.ir/paper/21051/en

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