COMPARISON OF OXACILLIN AGAR SCREENING AND PCR METHODS IN DETECTION OF METHICILLIN RESISTANCE STAPHYLOCOCCUS EPIDERMIDIS STRAINS ISOLATED FROM BLOOD CULTURES OF CHILDREN

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SHOJA S.; NAHAEI M.R.; AHANGARZADEH REZAEI MOHAMMAD; NIKVASH S.

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Journal: IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY Year:2008 | Volume:1 | Issue:4 Page(s): 13-20

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Title

COMPARISON OF OXACILLIN AGAR SCREENING AND PCR METHODS IN DETECTION OF METHICILLIN RESISTANCE STAPHYLOCOCCUS EPIDERMIDIS STRAINS ISOLATED FROM BLOOD CULTURES OF CHILDREN

Author(s)

SHOJA S. | NAHAEI M.R. | AHANGARZADEH REZAEI MOHAMMAD | NIKVASH S. | Issue Writer Certificate

Keywords

STAPHYLOCOCCUS EPIDERMIDISQ3

METHICILLINQ3

OXACILLIN SCREENING AGARQ4

MECA GENEQ3

Abstract

Background and Objectives: In recent years METHICILLIN resistant STAPHYLOCOCCUS EPIDERMIDIS is among the prevalent bacteria causing septicemia in neonates and children. Therefore the rapid diagnosis and their antibiotic resistance surveillance are of great importance for treatment. The aim of this study was to evaluate OXACILLIN SCREENING AGAR with three concentrations of oxacillin and to compare with PCR in detection of METHICILLIN resistant Staphyliococcus epidermidis.Material and Methods: A total of 100 STAPHYLOCOCCUS EPIDERMIDIS strains were recovered from blood cultures of neonates and children. Resistance of isolates to METHICILLIN was checked using oxacillin agar screening containing 0.6, 4, and 6 mg/ml of oxacillin. They were also tested for MECA GENE by PCR.Results: According to PCR results 89 of test isolates revealed to have MECA GENE. Oxacillin agar screening test containing 0.6 mg/ml oxacillin could detect 85 and 89 resistant strains after 24h and 48h incubation, respectively. The same test containing 4 mg/ml oxacillin could detect 78 and 89 resistant strains after 24h and 48h incubation, respectively and by using 6 mg/ml oxacillin 67 and 75 resistant strains detected after 24h and 48h incubation, respectively.Conclusion: Sensitivity of oxacillin agar screening test containing oxacillin concentrations of 0.6 mg/ml and 4 mg/ml after 48h incubation was the same as PCR test, whereas 6 mg/ml oxacillin showed low sensitivity in detection of resistant isolates. Therefore, oxacillin agar screening test with 0.6 mg/ml and 4 mg/ml after 48h incubation, which is a cheap and handy for any level of routine laboratory, could be used in accurate and effective detection of METHICILLIN resistant S. epidermidis (MRSE).

Cites

COMPARISON OF METHICILLIN RESISTANCE PHENOTYPE WITH MECA GENE CARRIAGE IN CLINICAL ISOLATES OF STAPHYLOCOCCUS EPIDERMIDIS

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APA: Copy

SHOJA, S., NAHAEI, M.R., AHANGARZADEH REZAEI, MOHAMMAD, & NIKVASH, S.. (2008). COMPARISON OF OXACILLIN AGAR SCREENING AND PCR METHODS IN DETECTION OF METHICILLIN RESISTANCE STAPHYLOCOCCUS EPIDERMIDIS STRAINS ISOLATED FROM BLOOD CULTURES OF CHILDREN. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, 1(4), 13-20. SID. https://sid.ir/paper/132531/en

Vancouver: Copy

SHOJA S., NAHAEI M.R., AHANGARZADEH REZAEI MOHAMMAD, NIKVASH S.. COMPARISON OF OXACILLIN AGAR SCREENING AND PCR METHODS IN DETECTION OF METHICILLIN RESISTANCE STAPHYLOCOCCUS EPIDERMIDIS STRAINS ISOLATED FROM BLOOD CULTURES OF CHILDREN. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY[Internet]. 2008;1(4):13-20. Available from: https://sid.ir/paper/132531/en

IEEE: Copy

S. SHOJA, M.R. NAHAEI, MOHAMMAD AHANGARZADEH REZAEI, and S. NIKVASH, “COMPARISON OF OXACILLIN AGAR SCREENING AND PCR METHODS IN DETECTION OF METHICILLIN RESISTANCE STAPHYLOCOCCUS EPIDERMIDIS STRAINS ISOLATED FROM BLOOD CULTURES OF CHILDREN,” IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, vol. 1, no. 4, pp. 13–20, 2008, [Online]. Available: https://sid.ir/paper/132531/en

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