OPTIMIZATION OF AMPLIFICATION AND EXTRACTION OF DNA FROM PARAFFIN EMBEDDED TISSUES BY A COMBINATORY PROCEDURE

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LOHRASBI NEJAD A.; YAGHOOBI M.M.; NAZEM H.

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ISSN: 2588-4824

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Paper Information

Journal: IRANIAN JOURNAL OF BIOLOGY Year:2010 | Volume:23 | Issue:1 Page(s): 132-140

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Title

OPTIMIZATION OF AMPLIFICATION AND EXTRACTION OF DNA FROM PARAFFIN EMBEDDED TISSUES BY A COMBINATORY PROCEDURE

Author(s)

LOHRASBI NEJAD A. | YAGHOOBI M.M. | NAZEM H. | Issue Writer Certificate

Keywords

PARAFFIN BLOCKQ3

DNA EXTRACTIONQ2

POLYMERASE CHAIN REACTIONQ2

CANCEROUS TISSUEQ3

Abstract

Paraffin embedded tissue found in archives of the pathology departments are valuable resources of human specimens for molecular studies of diseases such as cancer. However, fixation and parafination steps of tissue damage nucleic acids seriously and give rise to DNA with low quality, which is unsuitable for PCR method. In this research, we investigated some usual methods of extracting and amplifying DNA from paraffin-embedded tissue in order to identify and improve their weaknesses and propose a new combined method. The efficiency of different enzymes including Taq, pfu and pwo polymerases or their mixtures in amplifying DNA extracted from paraffin embedded tissues were also compared. The results shows the efficiency of combined method is higher than previous methods and gives DNA that could be amplified more during PCR reaction.

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APA: Copy

LOHRASBI NEJAD, A., YAGHOOBI, M.M., & NAZEM, H.. (2010). OPTIMIZATION OF AMPLIFICATION AND EXTRACTION OF DNA FROM PARAFFIN EMBEDDED TISSUES BY A COMBINATORY PROCEDURE. IRANIAN JOURNAL OF BIOLOGY, 23(1), 132-140. SID. https://sid.ir/paper/21056/en

Vancouver: Copy

LOHRASBI NEJAD A., YAGHOOBI M.M., NAZEM H.. OPTIMIZATION OF AMPLIFICATION AND EXTRACTION OF DNA FROM PARAFFIN EMBEDDED TISSUES BY A COMBINATORY PROCEDURE. IRANIAN JOURNAL OF BIOLOGY[Internet]. 2010;23(1):132-140. Available from: https://sid.ir/paper/21056/en

IEEE: Copy

A. LOHRASBI NEJAD, M.M. YAGHOOBI, and H. NAZEM, “OPTIMIZATION OF AMPLIFICATION AND EXTRACTION OF DNA FROM PARAFFIN EMBEDDED TISSUES BY A COMBINATORY PROCEDURE,” IRANIAN JOURNAL OF BIOLOGY, vol. 23, no. 1, pp. 132–140, 2010, [Online]. Available: https://sid.ir/paper/21056/en

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