CONSTRUCTION OF RECOMBINANT AUTOLYTIC E.COLI CELLS PRODUCING BIOPLASTIC VIA CLONING OF POLY(3 HYDROXYBUTYRATE) OPERON AND GENE E (E.LYSIS) COORDINATELY

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

GHAEDMOHAMMADI S.; SADEGHIZADEH M.; VASHEGHANI FARAHANI E.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF BIOLOGY Year:2010 | Volume:23 | Issue:4 Page(s): 498-507

Download Full-Text

Persian Verion

View:

981

Download:

Cites:

Information Journal Paper

Title

CONSTRUCTION OF RECOMBINANT AUTOLYTIC E.COLI CELLS PRODUCING BIOPLASTIC VIA CLONING OF POLY(3 HYDROXYBUTYRATE) OPERON AND GENE E (E.LYSIS) COORDINATELY

Author(s)

GHAEDMOHAMMADI S. | SADEGHIZADEH M. | VASHEGHANI FARAHANI E. | Issue Writer Certificate

Keywords

POLYHYDROXYBUTYRATEQ2

BIOPLASTICQ3

RALSTONIA EUTROPHAQ3

AUTOLYSISQ3

Abstract

Production of material such as synthetic polymers resistant to biodegradation increases wastes and accumulates them in environment for a long time. Hence, the production of biodegradable polymers particularly from renewable and natural resources become necessary. POLYHYDROXYBUTYRATE(PHB) is a storage material and biodegradable polymer in a great variety of bacteria. RALSTONIA EUTROPHA has been studied extensively because of its ability to accumulate PHB. In this bacteria PHB is synthesized by acetyl coA in three steps by function of three enzymes, b-ketothiolase, NADPH-dependent acetylcoA reductase, PHA synthase which are encoded by phbA, phbB, phbC, respectively. These genes are located in an operon. In this project, A strain of Escherichia coli was engineered to express poly(3 hydroxybutyrate) and gene E (E.lysis) coordinately. A gene encoding E.lysis was placed under the control of the IPTG-inducible T7 promoter in pET28a while the polyhydroxyalkanoate synthesis operon (phaCAB) from R. eutropha was expressed constitutively under the control of its native promoter. After the production of PHB in late logarithmic phase, IPTG was added and gene E expressed. The aim of this project is the construction of autolysing E. coli cells which produce BIOPLASTIC.

Cites

No record.

References

No record.

Cite

APA: Copy

GHAEDMOHAMMADI, S., SADEGHIZADEH, M., & VASHEGHANI FARAHANI, E.. (2010). CONSTRUCTION OF RECOMBINANT AUTOLYTIC E.COLI CELLS PRODUCING BIOPLASTIC VIA CLONING OF POLY(3 HYDROXYBUTYRATE) OPERON AND GENE E (E.LYSIS) COORDINATELY. IRANIAN JOURNAL OF BIOLOGY, 23(4), 498-507. SID. https://sid.ir/paper/21145/en

Vancouver: Copy

GHAEDMOHAMMADI S., SADEGHIZADEH M., VASHEGHANI FARAHANI E.. CONSTRUCTION OF RECOMBINANT AUTOLYTIC E.COLI CELLS PRODUCING BIOPLASTIC VIA CLONING OF POLY(3 HYDROXYBUTYRATE) OPERON AND GENE E (E.LYSIS) COORDINATELY. IRANIAN JOURNAL OF BIOLOGY[Internet]. 2010;23(4):498-507. Available from: https://sid.ir/paper/21145/en

IEEE: Copy

S. GHAEDMOHAMMADI, M. SADEGHIZADEH, and E. VASHEGHANI FARAHANI, “CONSTRUCTION OF RECOMBINANT AUTOLYTIC E.COLI CELLS PRODUCING BIOPLASTIC VIA CLONING OF POLY(3 HYDROXYBUTYRATE) OPERON AND GENE E (E.LYSIS) COORDINATELY,” IRANIAN JOURNAL OF BIOLOGY, vol. 23, no. 4, pp. 498–507, 2010, [Online]. Available: https://sid.ir/paper/21145/en

Related Journal Papers