CONSTRUCTION AND OVEREXPRESSION OF A NOVEL HYBRID PROTEIN CONSISTING OF ROTAVIRUS ENTEROTOXIN NSP4 AND SUBUNIT OF CS3 PILI IN E.COLI

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HOSSEIN S.A.; YAKHCHALI B.; SATARI MORTEZA; DASHTBAN M.

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Journal: IRANIAN JOURNAL OF BIOLOGY Year:2002 | Volume:12 | Issue:1-2 Page(s): 1-12

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Title

CONSTRUCTION AND OVEREXPRESSION OF A NOVEL HYBRID PROTEIN CONSISTING OF ROTAVIRUS ENTEROTOXIN NSP4 AND SUBUNIT OF CS3 PILI IN E.COLI

Author(s)

HOSSEIN S.A. | YAKHCHALI B. | SATARI MORTEZA | DASHTBAN M. | Issue Writer Certificate

Keywords

NSP4Q4

ROTAVIRUSQ4

CS3Q4

ETECQ2

E.COLIQ2

Abstract

Rotavirus infection is the major etiological agent of severe diarrhea in infant and young children. Nonstructural glycoprotein NSP4 of ROTAVIRUS and its epitope (amino acids 114-135). which act as enterotoxin are considered in vaccine development against ROTAVIRUS. Coli surface antigen 3 (CS3) pili of Enterotoxigenic Escherichia coli (ETEC) can be used as a system for expression of foreign antigenic determinants on the bacterial surface. In this study, cDNA encoding for NSP4114-135 was amplified by PCR and after digestion with endonuclease StyI was cloned in cstH gene encoding structural subunit of CS3 fimbriae. Recombinant plasmids were screened by PCR, restriction enzyme analysis, DNA hybridization and DNA sequencing. Hybrid gene, cstH:: NSP4114-135 was then cloned in the expression vector pET21a(+) under the control of T7 promoter. Expression of hybrid protein CSTH:: NSP4114-135 in E.COLI DH5α was indicated by SDS PAGE and Western blot analysis using antibodies against CS3 and NSP4. Expression of hybrid protein on the surface of E.COLI as chimeric pili and its immunogenicity are currently under investigation.

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APA: Copy

HOSSEIN, S.A., YAKHCHALI, B., SATARI, MORTEZA, & DASHTBAN, M.. (2002). CONSTRUCTION AND OVEREXPRESSION OF A NOVEL HYBRID PROTEIN CONSISTING OF ROTAVIRUS ENTEROTOXIN NSP4 AND SUBUNIT OF CS3 PILI IN E.COLI. IRANIAN JOURNAL OF BIOLOGY, 12(1-2), 1-12. SID. https://sid.ir/paper/21172/en

Vancouver: Copy

HOSSEIN S.A., YAKHCHALI B., SATARI MORTEZA, DASHTBAN M.. CONSTRUCTION AND OVEREXPRESSION OF A NOVEL HYBRID PROTEIN CONSISTING OF ROTAVIRUS ENTEROTOXIN NSP4 AND SUBUNIT OF CS3 PILI IN E.COLI. IRANIAN JOURNAL OF BIOLOGY[Internet]. 2002;12(1-2):1-12. Available from: https://sid.ir/paper/21172/en

IEEE: Copy

S.A. HOSSEIN, B. YAKHCHALI, MORTEZA SATARI, and M. DASHTBAN, “CONSTRUCTION AND OVEREXPRESSION OF A NOVEL HYBRID PROTEIN CONSISTING OF ROTAVIRUS ENTEROTOXIN NSP4 AND SUBUNIT OF CS3 PILI IN E.COLI,” IRANIAN JOURNAL OF BIOLOGY, vol. 12, no. 1-2, pp. 1–12, 2002, [Online]. Available: https://sid.ir/paper/21172/en

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