SOMATIC EMBRYOGENESIS OF WHEAT (TRITICUM AESTIVUM L. CV. HYRMAND) IN SOLID AND LIQUID MEDIA

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KIAROSTAMI KH.; EBRAHIMZADEH H.; MADJIDI HERVAN E.

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Journal: IRANIAN JOURNAL OF BIOLOGY Year:2002 | Volume:11 | Issue:3-4 Page(s): 61-72

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Title

SOMATIC EMBRYOGENESIS OF WHEAT (TRITICUM AESTIVUM L. CV. HYRMAND) IN SOLID AND LIQUID MEDIA

Author(s)

KIAROSTAMI KH. | EBRAHIMZADEH H. | MADJIDI HERVAN E. | Issue Writer Certificate

Keywords

TISSUE CULTUREQ2

EMBRYOGENESISQ2

CALLUSQ4

MEDIUMQ2

Abstract

Immature embryos of wheat (Triticum aestivum L. cv. Hyrmand) were cultured on MS MEDIUM with different concentration of 2, 4 –D, and embryogenic CALLUS formation were studied. Embryogenic calli were derived from immature embryos grown on MS MEDIUM with 1 mg/ L 2, 4 –D.Embroyogenic calli were subcultured in maintenance MEDIUM having 2 mg /L 2, 4-D, 100mg/L casein hydrolysate and 500 mg / L glutamine, and with different intervals transferred to liquid MEDIUM. These calli were investigated for potential of EMBRYOGENESIS. Analysis variance of data showed no significant differences in induction of EMBRYOGENESIS in response to different levels of the hormones. In these media, two main types of calli were formed: embryogenic, off white, compact and nodular CALLUS (type A) and non-embryogenic soft and watery CALLUS. Also a few white and compact embryogenic CALLUS (type B) were formed. Repeated subculture (5-8 month) of the embryogenic CALLUS were rise to a droughty, compact and nodular embryogenic CALLUS (type C), and with more subculture, droughty and friable embryogenic CALLUS (type D) were formed. Embryogenic calli obtained above were cultured in liquid MEDIUM in the presence of 2,4-D or ABA, and EMBRYOGENESIS were studied for 28 days of culturing in presence of 1 mg/ L 2,4 –D. After this period 1.8 mg/L of ABA was added to MS MEDIUM. ABA significantly increased embryogenic clumps. Callus clumps were transferred to solid hormone- free MEDIUM every two weeks. Various CALLUS types identified, some of them were embryogenic and some were non- embryogenic.

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APA: Copy

KIAROSTAMI, KH., EBRAHIMZADEH, H., & MADJIDI HERVAN, E.. (2002). SOMATIC EMBRYOGENESIS OF WHEAT (TRITICUM AESTIVUM L. CV. HYRMAND) IN SOLID AND LIQUID MEDIA. IRANIAN JOURNAL OF BIOLOGY, 11(3-4), 61-72. SID. https://sid.ir/paper/21242/en

Vancouver: Copy

KIAROSTAMI KH., EBRAHIMZADEH H., MADJIDI HERVAN E.. SOMATIC EMBRYOGENESIS OF WHEAT (TRITICUM AESTIVUM L. CV. HYRMAND) IN SOLID AND LIQUID MEDIA. IRANIAN JOURNAL OF BIOLOGY[Internet]. 2002;11(3-4):61-72. Available from: https://sid.ir/paper/21242/en

IEEE: Copy

KH. KIAROSTAMI, H. EBRAHIMZADEH, and E. MADJIDI HERVAN, “SOMATIC EMBRYOGENESIS OF WHEAT (TRITICUM AESTIVUM L. CV. HYRMAND) IN SOLID AND LIQUID MEDIA,” IRANIAN JOURNAL OF BIOLOGY, vol. 11, no. 3-4, pp. 61–72, 2002, [Online]. Available: https://sid.ir/paper/21242/en

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