PRODUCING OF EMBRYOGENIC CALLUS FROM ZYGOTIC EMBRYO IN DOREMA AMMONIACUM D.

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GHASEMIAN KHADIJEH; NAZERI SONBOL; CHEHREGANI RAD ABDOLKARIM; MIRZAIE ASL ASGHAR

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Journal: Journal of Plant Research Year:2015 | Volume:28 | Issue:2 Page(s): 364-373

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Title

PRODUCING OF EMBRYOGENIC CALLUS FROM ZYGOTIC EMBRYO IN DOREMA AMMONIACUM D.

Author(s)

GHASEMIAN KHADIJEH | NAZERI SONBOL | CHEHREGANI RAD ABDOLKARIM | MIRZAIE ASL ASGHAR | Issue Writer Certificate

Keywords

INDUCTION OF EMBRYOGENESISQ2

SOMATIC EMBRYOGENESISQ2

PLANT HORMONESQ1

Abstract

Dorema ammoniacum is a native medicinal plant in Iran, which due to ruinous harvesting practices and its natural habitats are destroyed in the country, the species is getting endangered. SOMATIC EMBRYOGENESIS is considered as useful method for propagation of plants. In the present study induction of embryogenic callus from zygotic embryo of this plant were investigated using MS and 1/2 MS basal media and also the different concentrations of NAA and BA hormones. It was observed that MS medium containing 1 mg/l NAA with 0.5 mg/l BA hormones was the best medium for callus induction and maximum number of somatic embryos production. Furthermore, MS basal medium compared to ½ MS basal medium showed a better result, not only in the callus formation but also in the SOMATIC EMBRYOGENESIS. No embryogenesis was observed after sub-culturing of non-embryogenic calluses in hormone-free MS basal medium. When embryos were transferred to media without growth regulators, whole plantlets were regenerated.

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APA: Copy

GHASEMIAN, KHADIJEH, NAZERI, SONBOL, CHEHREGANI RAD, ABDOLKARIM, & MIRZAIE ASL, ASGHAR. (2015). PRODUCING OF EMBRYOGENIC CALLUS FROM ZYGOTIC EMBRYO IN DOREMA AMMONIACUM D.. JOURNAL OF PLANT RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 28(2), 364-373. SID. https://sid.ir/paper/248942/en

Vancouver: Copy

GHASEMIAN KHADIJEH, NAZERI SONBOL, CHEHREGANI RAD ABDOLKARIM, MIRZAIE ASL ASGHAR. PRODUCING OF EMBRYOGENIC CALLUS FROM ZYGOTIC EMBRYO IN DOREMA AMMONIACUM D.. JOURNAL OF PLANT RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2015;28(2):364-373. Available from: https://sid.ir/paper/248942/en

IEEE: Copy

KHADIJEH GHASEMIAN, SONBOL NAZERI, ABDOLKARIM CHEHREGANI RAD, and ASGHAR MIRZAIE ASL, “PRODUCING OF EMBRYOGENIC CALLUS FROM ZYGOTIC EMBRYO IN DOREMA AMMONIACUM D.,” JOURNAL OF PLANT RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 28, no. 2, pp. 364–373, 2015, [Online]. Available: https://sid.ir/paper/248942/en

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