IDENTIFICATION OF CHROMATIN PROTEINS AND DNA REPEAT LENGHT IN SOLANUM TUBEROSUM

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

ARAMVASH A.; RABBANI CHADEGANI A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF BIOLOGY Year:2011 | Volume:23 | Issue:5 Page(s): 648-661

Download Full-Text

Persian Verion

View:

2,021

Download:

Cites:

Information Journal Paper

Title

IDENTIFICATION OF CHROMATIN PROTEINS AND DNA REPEAT LENGHT IN SOLANUM TUBEROSUM

Author(s)

ARAMVASH A. | RABBANI CHADEGANI A. | Issue Writer Certificate

Keywords

POTATOQ2

NUCLEOSOMEQ2

HISTONESQ3

NON-HISTONE PROTEINSQ3

ELECTROPHORESISQ2

Abstract

Chromatin is a complex of DNA, RNA, HISTONES and nonhistone chromosomal proteins. Identification of these proteins help us for better understanding of different mechanisms of gene expression. There are several methods for extraction of HISTONES and nonhistone proteins from animal cells, but less information of a comparable state is available for plants. Also, biochemical evidence has shown that the DNA repeats length in micrococcal nuclease digests of the chromatin of higher eukaryotes varies widely. In this study, POTATO leaf cells, cell suspensions and calli were extracted with different methods using, acids and salts. Then the proteins were analyzed on SDS polyacrylamide gel ELECTROPHORESIS and immunoblotted against HISTONES and HMG antisera. The results show that HISTONES H3 and H4 are relatively the same as their animal counterparts but H2A and H2B in POTATO are larger than their animal counterparts and exist as a several molecular weight variants. Also HMG proteins exhibit four main proteins in total tissue extract POTATO leaves and their electrophoretic pattern is different from mammalian HMG proteins. The POTATO leaf HMG proteins could not be detected by immunoblotting against thymus HMG antisera. Examination of extensive Micrococcal nuclease digest suggests that the DNA repeat length is approximately 158 base pairs which are shorter than the DNA repeat length of mammals and also other plants. It is concluded that there is little homology between the chromatin proteins and DNA repeat length of the animals and POTATO leaf cells.

Cites

No record.

References

No record.

Cite

APA: Copy

ARAMVASH, A., & RABBANI CHADEGANI, A.. (2011). IDENTIFICATION OF CHROMATIN PROTEINS AND DNA REPEAT LENGHT IN SOLANUM TUBEROSUM. IRANIAN JOURNAL OF BIOLOGY, 23(5), 648-661. SID. https://sid.ir/paper/21306/en

Vancouver: Copy

ARAMVASH A., RABBANI CHADEGANI A.. IDENTIFICATION OF CHROMATIN PROTEINS AND DNA REPEAT LENGHT IN SOLANUM TUBEROSUM. IRANIAN JOURNAL OF BIOLOGY[Internet]. 2011;23(5):648-661. Available from: https://sid.ir/paper/21306/en

IEEE: Copy

A. ARAMVASH, and A. RABBANI CHADEGANI, “IDENTIFICATION OF CHROMATIN PROTEINS AND DNA REPEAT LENGHT IN SOLANUM TUBEROSUM,” IRANIAN JOURNAL OF BIOLOGY, vol. 23, no. 5, pp. 648–661, 2011, [Online]. Available: https://sid.ir/paper/21306/en

Related Journal Papers