SIMULTANEOUS DETECTION OF CAGA GENE AND VACA ALLELES IN HELICOBACTER PYLORI ISOLATED FROM PATIENTS WITH GASTROINTESTINAL INFLAMMATIONS USING MULTIPLEX PCR.

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MOBARAKI M.; ROGHANIAN R.; AZARM T.; ZARKESH ESFAHANI S.H.; DAGHAGHZADEH H.

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Journal: IRANIAN JOURNAL OF BIOLOGY Year:2011 | Volume:24 | Issue:6 Page(s): 895-903

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Title

SIMULTANEOUS DETECTION OF CAGA GENE AND VACA ALLELES IN HELICOBACTER PYLORI ISOLATED FROM PATIENTS WITH GASTROINTESTINAL INFLAMMATIONS USING MULTIPLEX PCR.

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Keywords

HELICOBACTER PYLORIQ2

MULTIPLEX PCRQ2

CAGA GENE AND VACA ALLELESQ2

GASTROINTESTINAL INFLAMMATIONQ2

Abstract

HELICOBACTER PYLORIis a gram negative and microaerophilic bacterium which infects a lot of people all over the world. Although in most cases the infected individuals usually show no symptoms of infection, but seldom its infection leads to GASTROINTESTINAL INFLAMMATION, peptic ulcer and even malignancies such as gastric adenocarcinoma and lymphoma. Several virulence factors of this bacterium including cagA and vacA adequately have been investigated by numerous researchers in this field. The aim of this study was to establish the MULTIPLEX PCR test for a simultaneous and rapid detection of the above mentioned genes as well as determining any relationship between the virulence factors and the severity of the GASTROINTESTINAL INFLAMMATIONs. In this study, gastric biopsies of 66 patients with positive rapid urease test were collected for further studies. From these samples, only 21 could grow on standard culture media. All the bacterial isolates were confirmed by biochemical tests. Chromosomal DNA was extracted from bacterial isolates and further examined by MULTIPLEX PCR. In 15 cases cagA, 9 cases s1m1, 12 cases s1m2 and 5 cases s2m2 were seen. No s2m1 genotype was found. The most frequent allelic forms were related to s1 and m2 alleles. Most of the patients in this study had diffuse antral gastritis. In most cases cagA and s1m2 were accompanied together. These data indicate that probably there is a relationship between simultaneous presence of these factors and the severity of the GASTROINTESTINAL INFLAMMATIONs.

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APA: Copy

MOBARAKI, M., ROGHANIAN, R., AZARM, T., ZARKESH ESFAHANI, S.H., & DAGHAGHZADEH, H.. (2011). SIMULTANEOUS DETECTION OF CAGA GENE AND VACA ALLELES IN HELICOBACTER PYLORI ISOLATED FROM PATIENTS WITH GASTROINTESTINAL INFLAMMATIONS USING MULTIPLEX PCR.. IRANIAN JOURNAL OF BIOLOGY, 24(6), 895-903. SID. https://sid.ir/paper/21329/en

Vancouver: Copy

MOBARAKI M., ROGHANIAN R., AZARM T., ZARKESH ESFAHANI S.H., DAGHAGHZADEH H.. SIMULTANEOUS DETECTION OF CAGA GENE AND VACA ALLELES IN HELICOBACTER PYLORI ISOLATED FROM PATIENTS WITH GASTROINTESTINAL INFLAMMATIONS USING MULTIPLEX PCR.. IRANIAN JOURNAL OF BIOLOGY[Internet]. 2011;24(6):895-903. Available from: https://sid.ir/paper/21329/en

IEEE: Copy

M. MOBARAKI, R. ROGHANIAN, T. AZARM, S.H. ZARKESH ESFAHANI, and H. DAGHAGHZADEH, “SIMULTANEOUS DETECTION OF CAGA GENE AND VACA ALLELES IN HELICOBACTER PYLORI ISOLATED FROM PATIENTS WITH GASTROINTESTINAL INFLAMMATIONS USING MULTIPLEX PCR.,” IRANIAN JOURNAL OF BIOLOGY, vol. 24, no. 6, pp. 895–903, 2011, [Online]. Available: https://sid.ir/paper/21329/en

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