ISOLATION AND IDENTIFICATION OF ETHANOL PRODUCER YEASTS FROM D-XYLOSE

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HAMIDI MOTLAGH R.A.; NAHVI I.; EMTIAZI G.; GHEZELBASH GH.R.

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Journal: IRANIAN JOURNAL OF BIOLOGY Year:2009 | Volume:22 | Issue:1 Page(s): 46-52

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Title

ISOLATION AND IDENTIFICATION OF ETHANOL PRODUCER YEASTS FROM D-XYLOSE

Author(s)

HAMIDI MOTLAGH R.A. | NAHVI I. | EMTIAZI G. | GHEZELBASH GH.R. | Issue Writer Certificate

Keywords

ETHANOLQ2

XYLOSEQ2

FERMENTATIONQ2

C. BOIDINIIQ3

Abstract

D-xylose, is the second most abundant sugar in nature and it is one of the main constituents of plants. Hence, ETHANOL production from D-xylose has an ignorable importance in the world future energy trends. In this research, natural environments and materials such as compost and decaying body of plants and agricultural wastes, were investigated for finding XYLOSE fermentating yeasts, which can produce ETHANOL using D-xylose. First, one gram of different samples were cultured in broth culture media comprising XYLOSE as the only energy source and yeast extract and after 24 hours 0.1 ml of brothes were transferred on solid medium containing the same elements. D-xylose utilizing yeasts were examined for their ability to ferment D-xylose using their culture in mild agitation. From 61 isolates, 5 isolates could utilize D-xylose and produce less than 1 g/l ETHANOL from 20 g/l D-xylose and 7 isolates could produce more than 1 g/l ETHANOL from 20 g/l D-xylose. These later isolates were Candida boidinii, Candida paludigena, three isolates of Kluyveromyces marxianus and two isolates of Auerobasidium polullans. Among these isolates, Candida boidinii with 5.8 g/l ETHANOL from 20 g/l D-xylose was the best ETHANOL producer. As a result of this study, 100 rpm agitation, 5 g/l yeast extract, 60-84 hours incubation time and pH=4.5 were the best environmental condition for the maximum production.

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APA: Copy

HAMIDI MOTLAGH, R.A., NAHVI, I., EMTIAZI, G., & GHEZELBASH, GH.R.. (2009). ISOLATION AND IDENTIFICATION OF ETHANOL PRODUCER YEASTS FROM D-XYLOSE. IRANIAN JOURNAL OF BIOLOGY, 22(1), 46-52. SID. https://sid.ir/paper/21412/en

Vancouver: Copy

HAMIDI MOTLAGH R.A., NAHVI I., EMTIAZI G., GHEZELBASH GH.R.. ISOLATION AND IDENTIFICATION OF ETHANOL PRODUCER YEASTS FROM D-XYLOSE. IRANIAN JOURNAL OF BIOLOGY[Internet]. 2009;22(1):46-52. Available from: https://sid.ir/paper/21412/en

IEEE: Copy

R.A. HAMIDI MOTLAGH, I. NAHVI, G. EMTIAZI, and GH.R. GHEZELBASH, “ISOLATION AND IDENTIFICATION OF ETHANOL PRODUCER YEASTS FROM D-XYLOSE,” IRANIAN JOURNAL OF BIOLOGY, vol. 22, no. 1, pp. 46–52, 2009, [Online]. Available: https://sid.ir/paper/21412/en

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