EFFECT OF DIFFERENT ENVIRONMENTAL FACTORS ON THE PRODUCTION OF THE PROTEINASE ASPARTIC ENZYME IN THE MUCOR SPECIES OF MOLD

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NAHVI I.; BALALI GH.; MOBINI DEHKORDI M.; SHAREGHI B.

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Journal: IRANIAN JOURNAL OF BIOLOGY Year:2001 | Volume:10 | Issue:1-2 Page(s): 1-11

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Title

EFFECT OF DIFFERENT ENVIRONMENTAL FACTORS ON THE PRODUCTION OF THE PROTEINASE ASPARTIC ENZYME IN THE MUCOR SPECIES OF MOLD

Author(s)

NAHVI I. | BALALI GH. | MOBINI DEHKORDI M. | SHAREGHI B. | Issue Writer Certificate

Keywords

PROTEINASE ASPARTICQ4

MUCORQ3

RENNETQ4

Abstract

The PROTEINASE ASPARTIC enzyme is produced by many species of fungi among which Zygomycetes are of special importance. Many species of fungi belonging to the genus MUCOR were isolated, purified and identified from different sources: dairy industries, different soils, different cheeses contaminated with fungi, composts and plants contaminated with fungi have been transferred to a soil medium containing %(w/w) of wheat bran for storing these molds, and for spore production they were also added to a PDA medium as an inoculum. Enzyme production was achieved in the culture medium containing wheat bran and water following solid base fermentation and enzyme solutions were obtained through the addition of citrate buffer to the culture medium followed by filtration. Enzyme activity and its coagulation ability were calculated for each case and the ratios of coagulating ability to proteolysis were determined. The highest ratio belonged to a species of MUCOR which was isolated from soil. In the continuation of the experiment, for increasing the level of enzyme production, and its coagulation ability, the effects of different treatments on the above mentioned fungus were studied. The highest ratio of coagulating ability to proteolysis was obtained through the inoculation of 700000 spores to each gram of wheat bran culture medium. Ammonium chloride and yeast extract can stimulate enzyme production with optimum amounts of 1% and 1.5% respectively. Since the concerning mould was isolated from the soil, the optimum incubation temperature for the production of the enzyme has been determined as 27ºC. Enzyme production reached its maximum after 72 hours of incubation and the best pH for the production of the enzyme was determined to be 6.5. During enzyme amount the activity of the enzyme was increased when pH 6 enzyme solution. The addition of 8.9 ml of whey with a pH of 6.5 and sugar beet molasses with a pH of 6.5 and brix of 26 to the culture medium increased the enzyme production 2.93% and 5.1% respectively.

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APA: Copy

NAHVI, I., BALALI, GH., MOBINI DEHKORDI, M., & SHAREGHI, B.. (2001). EFFECT OF DIFFERENT ENVIRONMENTAL FACTORS ON THE PRODUCTION OF THE PROTEINASE ASPARTIC ENZYME IN THE MUCOR SPECIES OF MOLD . IRANIAN JOURNAL OF BIOLOGY, 10(1-2), 1-11. SID. https://sid.ir/paper/21475/en

Vancouver: Copy

NAHVI I., BALALI GH., MOBINI DEHKORDI M., SHAREGHI B.. EFFECT OF DIFFERENT ENVIRONMENTAL FACTORS ON THE PRODUCTION OF THE PROTEINASE ASPARTIC ENZYME IN THE MUCOR SPECIES OF MOLD . IRANIAN JOURNAL OF BIOLOGY[Internet]. 2001;10(1-2):1-11. Available from: https://sid.ir/paper/21475/en

IEEE: Copy

I. NAHVI, GH. BALALI, M. MOBINI DEHKORDI, and B. SHAREGHI, “EFFECT OF DIFFERENT ENVIRONMENTAL FACTORS ON THE PRODUCTION OF THE PROTEINASE ASPARTIC ENZYME IN THE MUCOR SPECIES OF MOLD ,” IRANIAN JOURNAL OF BIOLOGY, vol. 10, no. 1-2, pp. 1–11, 2001, [Online]. Available: https://sid.ir/paper/21475/en

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