EFFECT OF CORTISOL ON PHAGOCYTIC ACTIVITY OF MACROPHAGES IN RAINBOW TROUT

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VAHDATI A.; NEKUI N.; ADIB M.

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ISSN: 2588-4824

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Journal: IRANIAN JOURNAL OF BIOLOGY Year:2001 | Volume:10 | Issue:1-2 Page(s): 12-21

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Title

EFFECT OF CORTISOL ON PHAGOCYTIC ACTIVITY OF MACROPHAGES IN RAINBOW TROUT

Author(s)

VAHDATI A. | NEKUI N. | ADIB M. | Issue Writer Certificate

Keywords

CORTISOLQ2

PHAGOCYTOSISQ4

RAINBOW TROUT MACROPHAGESQ4

Abstract

CORTISOL, in addition to its metabolic effects, influences the immunity mechanisms. Macrophages play a critical role in the production of O2, H2O2, cytokines, changes in lysosomal enzymes and also PHAGOCYTOSIS. The aim of the present study is to investigate the CORTISOL effects on the phagocytic activity of macrophages in rainbow trout in vitro. For this purpose the macrophages from pronepheros were removed into a Leibovitz medium. Then suspension were flooded onto microscope slides and allowed the phagocytic cells to adhere for 90 min. by 21˚C. After washing the slides by phosphate buffer solution (pH 7.8), the attached macrophages were flooded with yeast cells suspension. PHAGOCYTOSIS was allowed to proceed for 90 min. After staining the average of engulfed yeast cells by a macrophage was determined, inspecting 200 macrophaged. The present results showed that the incubation of macrophages with CORTISOL depressed the PHAGOCYTOSIS after 3h and incubation for a period less than 3h had no significant effect. This study showed, that CORTISOL acts directly on macrophages and its effect id slow and time dependent

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APA: Copy

VAHDATI, A., NEKUI, N., & ADIB, M.. (2001). EFFECT OF CORTISOL ON PHAGOCYTIC ACTIVITY OF MACROPHAGES IN RAINBOW TROUT . IRANIAN JOURNAL OF BIOLOGY, 10(1-2), 12-21. SID. https://sid.ir/paper/21478/en

Vancouver: Copy

VAHDATI A., NEKUI N., ADIB M.. EFFECT OF CORTISOL ON PHAGOCYTIC ACTIVITY OF MACROPHAGES IN RAINBOW TROUT . IRANIAN JOURNAL OF BIOLOGY[Internet]. 2001;10(1-2):12-21. Available from: https://sid.ir/paper/21478/en

IEEE: Copy

A. VAHDATI, N. NEKUI, and M. ADIB, “EFFECT OF CORTISOL ON PHAGOCYTIC ACTIVITY OF MACROPHAGES IN RAINBOW TROUT ,” IRANIAN JOURNAL OF BIOLOGY, vol. 10, no. 1-2, pp. 12–21, 2001, [Online]. Available: https://sid.ir/paper/21478/en

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