EVALUATION OF OPTIMAL CONDITIONS FOR THE MEASURING OF PHAGOCYTIC ACTIVITY OF GRANULOCYTES IN RAINBOW TROUT (ONCORHYNCHUS MYKISS)

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VAHDATI A.; RAHIMI H.; MADANI H.; FAZELI A.

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Journal: Journal of Applied Biology Year:2001 | Volume:14 | Issue:1 Page(s): 15-21

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Title

EVALUATION OF OPTIMAL CONDITIONS FOR THE MEASURING OF PHAGOCYTIC ACTIVITY OF GRANULOCYTES IN RAINBOW TROUT (ONCORHYNCHUS MYKISS)

Author(s)

VAHDATI A. | RAHIMI H. | MADANI H. | FAZELI A. | Issue Writer Certificate

Keywords

GRANULOCYTESQ4

PHAGOCYTIC ACTIVITYQ4

RAINBOW TROUT (ONCORHYNCHUS MYKISS).Q4

Abstract

In order to assay PHAGOCYTIC ACTIVITY of PMNs of rainbow trout these cells were separated from blood and an appropriate concentration of PMNs or GRANULOCYTES was mixed with yeast cells at a certain ratio on the slide. The ingestion of yeast cells by PMNs was assayed by microscope. For measuring PHAGOCYTIC ACTIVITY two prameters were used: 1) The percentage of PHAGOCYTIC ACTIVITY (PA): This parameter was determined according to the following method: P A = (number of phagocytizing PMNs/ number of total cells) × 100 2) Phagocytic index (PI): This was assayed by counting the average number of ingested yeast cells by each PMN cell, in a 300 cell population. In this survey, the effect of factors such as incubation time, fresh rainbow trout serum (RTS) and fetal calf serum (FCS) were studied on PHAGOCYTIC ACTIVITY and it was measured. The obtained results indicated that percentage of PHAGOCYTIC ACTIVITY in 30 to 120 minutes is increased from 29.9 to 71.3 while there was no significant alteration in: phagocytic index. Fresh RTS had no effective significant change on PA and PI. In culture supplemented with FCS, the percentage of detected phagocytic cells were increased, while the number of yeast cells engulfed by each cell were no significantly altered.

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APA: Copy

VAHDATI, A., RAHIMI, H., MADANI, H., & FAZELI, A.. (2001). EVALUATION OF OPTIMAL CONDITIONS FOR THE MEASURING OF PHAGOCYTIC ACTIVITY OF GRANULOCYTES IN RAINBOW TROUT (ONCORHYNCHUS MYKISS). JOURNAL OF APPLIED BIOLOGY (JOURNAL OF SCIENCE), 14(1), 15-21. SID. https://sid.ir/paper/22678/en

Vancouver: Copy

VAHDATI A., RAHIMI H., MADANI H., FAZELI A.. EVALUATION OF OPTIMAL CONDITIONS FOR THE MEASURING OF PHAGOCYTIC ACTIVITY OF GRANULOCYTES IN RAINBOW TROUT (ONCORHYNCHUS MYKISS). JOURNAL OF APPLIED BIOLOGY (JOURNAL OF SCIENCE)[Internet]. 2001;14(1):15-21. Available from: https://sid.ir/paper/22678/en

IEEE: Copy

A. VAHDATI, H. RAHIMI, H. MADANI, and A. FAZELI, “EVALUATION OF OPTIMAL CONDITIONS FOR THE MEASURING OF PHAGOCYTIC ACTIVITY OF GRANULOCYTES IN RAINBOW TROUT (ONCORHYNCHUS MYKISS),” JOURNAL OF APPLIED BIOLOGY (JOURNAL OF SCIENCE), vol. 14, no. 1, pp. 15–21, 2001, [Online]. Available: https://sid.ir/paper/22678/en

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