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Information Journal Paper

Title

PLASMID PROFILE STUDY AND DETERMINATION OF GENETICAL ORIGIN OF MULTIRESISTANCE IN 10 CHOSEN STRAINS OF ESCHERICHIA COLI

Pages

  22-33

Abstract

 Bacterial resistance to antibiotics and other physical and chemical antimicrobial agents generally results from extra-chromosomal DNA (such as plasmids), which usually carry several genes that are responsible for the resistance. In this research 100 strains of E. coli isolated from clinical samples were tested for resistance to antimicrobial agents, such as the antibiotics, heavy metals and UV ray and their MIC and MBC were determined, Then 10 strains with high multiresistance were chosen. For determination of genetical origin of resistance in these bacteria the plasmid profile of them were studied. After the plasmid profile detection (in number and size), ETHIDIUM BROMIDE was used for plasmid curing, after plasmid elimination, rate of resistance was detected and original strain and cured bacteria were compared, which indicated that the resistance decreased dramatically following plasmid curing. Thus, genetical origin of resistance can be attributed to plasmids. To confirm these results another experiment was designed by TRANSFORMATION of these plasmids into sensitive plasmid-free recipient bacterium (E. coli HB101). The results showed that in most strains, antibiotics, heavy metals and UV resistances were plasmid-mediated.

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    APA: Copy

    ZAMANI, I., KASRA KERMANSHAHI, R., & EMTIAZI, G.. (2007). PLASMID PROFILE STUDY AND DETERMINATION OF GENETICAL ORIGIN OF MULTIRESISTANCE IN 10 CHOSEN STRAINS OF ESCHERICHIA COLI. IRANIAN JOURNAL OF BIOLOGY, 20(1), 22-33. SID. https://sid.ir/paper/21483/en

    Vancouver: Copy

    ZAMANI I., KASRA KERMANSHAHI R., EMTIAZI G.. PLASMID PROFILE STUDY AND DETERMINATION OF GENETICAL ORIGIN OF MULTIRESISTANCE IN 10 CHOSEN STRAINS OF ESCHERICHIA COLI. IRANIAN JOURNAL OF BIOLOGY[Internet]. 2007;20(1):22-33. Available from: https://sid.ir/paper/21483/en

    IEEE: Copy

    I. ZAMANI, R. KASRA KERMANSHAHI, and G. EMTIAZI, “PLASMID PROFILE STUDY AND DETERMINATION OF GENETICAL ORIGIN OF MULTIRESISTANCE IN 10 CHOSEN STRAINS OF ESCHERICHIA COLI,” IRANIAN JOURNAL OF BIOLOGY, vol. 20, no. 1, pp. 22–33, 2007, [Online]. Available: https://sid.ir/paper/21483/en

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