STUDY OF APOPTOSIS AND NECROSIS INDUCED BY PECTIC ACID IN GH3/B6 RAT PITUITARY CELL LINE

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ATTARI F.; SEPEHRI H.; AJDARI S.; GOLIAEI B.; DELPHI L.

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Journal: IRANIAN JOURNAL OF BIOLOGY Year:2009 | Volume:22 | Issue:3 Page(s): 498-505

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Title

STUDY OF APOPTOSIS AND NECROSIS INDUCED BY PECTIC ACID IN GH3/B6 RAT PITUITARY CELL LINE

Author(s)

Keywords

PECTINQ2

APOPTOSISQ1

GH3/B6 CELLSQ3

NECTOSISQ3

Abstract

Two distinct modes of cell death, APOPTOSIS and necrosis can be distinguished based on differences in morphological, biochemical and molecular changes of dying cells. APOPTOSIS is the most common form of eukaryotic cell death. It is a physiological suicide mechanism that preserves homeostasis. It has been reported that dietary fibers such as PECTINs causes cell death either in vitro or in vivo. In this study the effects of the pectic acid (derived from apple) on the GH3/B6 cell death is investigated. Materials and methods: GH3/B6 CELLS were cultured in the Ham’s F12 medium enriched with 15% horse serum and 2.5% fetal bovine serum for 3 days. Then they were treated by various amounts of pectic acid in different periods (6, 24, 48 hours). Previous studies showed that bromocriptine is one of the apoptotic agents in these cells, so it was used as positive control. The cell viability was detected by MTT exclusive test. The nuclear morphology of cells was explored by florescent stains including Acridine Orange/Ethidium Bromide (AO/EB). In addition, percentage of necrotic and apoptotic cells were studied with TUNEL kit. MTT test has shown that different concentration of pectic acid in 24 h decreased GH3/B6 CELLS viability in a dose dependant manner. Furthermore, florescent staining and TUNEL test showed that pectic acid concentration from 100mg/ml up to 1mg/ml concentration can induce APOPTOSIS in a dose dependent manner and in the higher concentration such as 2.5 mg/ml and 5 mg/ml can induce necrosis in these cells (p<0.001). According to our results it seems that pectic acid causes APOPTOSIS and necrosis in GH3/B6 CELLS.

Cites

EFFECT OF PECTIC SUBSTANCES IN INDUCTION OF APOPTOSIS IN HUMAN PROSTATE CELL LINE DU145

References

THE EFFECT OF PECTIC ACID ON THE MORPHOLOGY, SURVIVAL AND FUNCTIONAL CHARACTERISTICS OF GH3/B6 CELLS

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APA: Copy

ATTARI, F., SEPEHRI, H., AJDARI, S., GOLIAEI, B., & DELPHI, L.. (2009). STUDY OF APOPTOSIS AND NECROSIS INDUCED BY PECTIC ACID IN GH3/B6 RAT PITUITARY CELL LINE. IRANIAN JOURNAL OF BIOLOGY, 22(3), 498-505. SID. https://sid.ir/paper/21556/en

Vancouver: Copy

ATTARI F., SEPEHRI H., AJDARI S., GOLIAEI B., DELPHI L.. STUDY OF APOPTOSIS AND NECROSIS INDUCED BY PECTIC ACID IN GH3/B6 RAT PITUITARY CELL LINE. IRANIAN JOURNAL OF BIOLOGY[Internet]. 2009;22(3):498-505. Available from: https://sid.ir/paper/21556/en

IEEE: Copy

F. ATTARI, H. SEPEHRI, S. AJDARI, B. GOLIAEI, and L. DELPHI, “STUDY OF APOPTOSIS AND NECROSIS INDUCED BY PECTIC ACID IN GH3/B6 RAT PITUITARY CELL LINE,” IRANIAN JOURNAL OF BIOLOGY, vol. 22, no. 3, pp. 498–505, 2009, [Online]. Available: https://sid.ir/paper/21556/en

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