EFFECT OF PECTIC SUBSTANCES IN INDUCTION OF APOPTOSIS IN HUMAN PROSTATE CELL LINE DU145

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

DASHT BOZORGI S.; SEPEHRI H.; GOLIAE B.; DELPHI L.; ZAMIN E.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Molecular and Cellular Researches Year:2013 | Volume:26 | Issue:2 Page(s): 186-199

Download Full-Text

Persian Verion

View:

842

Download:

Cites:

Information Journal Paper

Title

EFFECT OF PECTIC SUBSTANCES IN INDUCTION OF APOPTOSIS IN HUMAN PROSTATE CELL LINE DU145

Author(s)

Keywords

APOPTOSISQ1

ACID PECTICQ2

CITRUS PECTINQ2

MODIFIED CITRUS PECTINQ2

DU145 CELL LINEQ2

Abstract

Pectic substances are complex polysaccharides, rich in galactoside residues, capable of combining with the carbohydrate-binding domain of galectin-3. It has been shown that these biomaterials can induce APOPTOSIS in tumurigenic cells, possibly via interaction with galectin-3. In the present study the apoptotic effect of pectic acid (AP) and CITRUS PECTIN (CP) on the human prostate cancer cells DU145, which express galectin-3, is investigated. PA and CP were modified by alteration in the temperature and pH, and the apoptotic effect of such modified CP (MCP) and AP (MAP) was studied. We also used commercially available modified CITRUS PECTIN, Pectasol, as positive control. DU145 cells were treated by different concentration of AP, MAP, CP, MCP, and Pectasol in periods of 24 and 48 hours. The percentage of apoptotic cells, after these treatments were investigated using Acridine orange/ Ethidium bromide staining and Cell cycle analysis. These investigations showed that AP, MAP, MCP can induce high percentage of APOPTOSIS compared to control group (p<0.001). In addition, fluorescent staining showed that percentage of apoptotic cells were higher than necrotic cells. We demonstrated that, AP without any modification induced APOPTOSIS, yet CP should be modified to have such value. It may be a dominant point for AP as an edible pectin, while CITRUS PECTINase is in the non-edible peal and should be modified to have such property.

Cites

No record.

References

Cite

APA: Copy

DASHT BOZORGI, S., SEPEHRI, H., GOLIAE, B., DELPHI, L., & ZAMIN, E.. (2013). EFFECT OF PECTIC SUBSTANCES IN INDUCTION OF APOPTOSIS IN HUMAN PROSTATE CELL LINE DU145. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 26(2), 186-199. SID. https://sid.ir/paper/248587/en

Vancouver: Copy

DASHT BOZORGI S., SEPEHRI H., GOLIAE B., DELPHI L., ZAMIN E.. EFFECT OF PECTIC SUBSTANCES IN INDUCTION OF APOPTOSIS IN HUMAN PROSTATE CELL LINE DU145. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2013;26(2):186-199. Available from: https://sid.ir/paper/248587/en

IEEE: Copy

S. DASHT BOZORGI, H. SEPEHRI, B. GOLIAE, L. DELPHI, and E. ZAMIN, “EFFECT OF PECTIC SUBSTANCES IN INDUCTION OF APOPTOSIS IN HUMAN PROSTATE CELL LINE DU145,” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 26, no. 2, pp. 186–199, 2013, [Online]. Available: https://sid.ir/paper/248587/en

Related Journal Papers