THE USAGE OF AGRICULTURAL LIGNOCELLULOSIC WASTES IN COST- EFFECTIVE PRODUCTION AND OPTIMIZATION OF ALKALINE XYLANASE, BY AN INDIGENOUS STRAIN OF BACILLUS MOJAVENSIS THROUGH SUBMERGED FERMENTATION

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AKHAVAN SEPAHY A.; GHAZI SH.; JAFARIAGDAM J.; AKAVAN SEPAHY M.

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Paper Information

Journal: IRANIAN JOURNAL OF BIOLOGY Year:2011 | Volume:24 | Issue:3 Page(s): 328-341

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Title

THE USAGE OF AGRICULTURAL LIGNOCELLULOSIC WASTES IN COST- EFFECTIVE PRODUCTION AND OPTIMIZATION OF ALKALINE XYLANASE, BY AN INDIGENOUS STRAIN OF BACILLUS MOJAVENSIS THROUGH SUBMERGED FERMENTATION

Author(s)

AKHAVAN SEPAHY A. | GHAZI SH. | JAFARIAGDAM J. | AKAVAN SEPAHY M. | Issue Writer Certificate

Keywords

BACILLUS MJAVENSISQ2

OAT HUSKQ2

XYLANASEQ3

Abstract

In this project, an appropriate indigenous Bacillus strain for XYLANASE production was isolated from cotton farm (Iran- kashan). According to evaluation by preliminary and secondary screening procedures, the competency of produced XYLANASE by this strain was confirmed. pH and temperature of enzyme activity in elevated pH and temperature ranges was assessed by measurement of released amounts of reducing sugar method. Optimum pH 9 and temperature of 55 oC for XYLANASE activity for this strain determined as optimum points. 16S rRNA sequencing analysis identified the isolate, as a strain of Bacillus mojavensis. Optimization step for screened Bacillus mojavensis strain, as a superior strain in the course of enzyme economical production process was performed in the presence of molasses, bagasse, wheat bran, OAT HUSK and rice straw, and enzyme production level was evaluated. Finally, XYLANASE production level from 194/68 IU/ml, under non-optimized culture condition, increased to 302/466 IU/ml in optimized fermentation condition. Increase in cost- effective XYLANASE production by lignocellulosic wastes (Oat husk as optimum carbon source), strain ability to ferment such wastes, production of high level of XYLANASE, and optimum enzyme activity in alkaline pH and elevated temperature, all reveal the superiority of this Bacillus as a candidate for industrial-scale economical production of XYLANASE.

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APA: Copy

AKHAVAN SEPAHY, A., GHAZI, SH., JAFARIAGDAM, J., & AKAVAN SEPAHY, M.. (2011). THE USAGE OF AGRICULTURAL LIGNOCELLULOSIC WASTES IN COST- EFFECTIVE PRODUCTION AND OPTIMIZATION OF ALKALINE XYLANASE, BY AN INDIGENOUS STRAIN OF BACILLUS MOJAVENSIS THROUGH SUBMERGED FERMENTATION. IRANIAN JOURNAL OF BIOLOGY, 24(3), 328-341. SID. https://sid.ir/paper/21588/en

Vancouver: Copy

AKHAVAN SEPAHY A., GHAZI SH., JAFARIAGDAM J., AKAVAN SEPAHY M.. THE USAGE OF AGRICULTURAL LIGNOCELLULOSIC WASTES IN COST- EFFECTIVE PRODUCTION AND OPTIMIZATION OF ALKALINE XYLANASE, BY AN INDIGENOUS STRAIN OF BACILLUS MOJAVENSIS THROUGH SUBMERGED FERMENTATION. IRANIAN JOURNAL OF BIOLOGY[Internet]. 2011;24(3):328-341. Available from: https://sid.ir/paper/21588/en

IEEE: Copy

A. AKHAVAN SEPAHY, SH. GHAZI, J. JAFARIAGDAM, and M. AKAVAN SEPAHY, “THE USAGE OF AGRICULTURAL LIGNOCELLULOSIC WASTES IN COST- EFFECTIVE PRODUCTION AND OPTIMIZATION OF ALKALINE XYLANASE, BY AN INDIGENOUS STRAIN OF BACILLUS MOJAVENSIS THROUGH SUBMERGED FERMENTATION,” IRANIAN JOURNAL OF BIOLOGY, vol. 24, no. 3, pp. 328–341, 2011, [Online]. Available: https://sid.ir/paper/21588/en

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