TOBACCO ANTIOXIDANT ENZYMES INVOLVEMENT IN RESISTANCE TO THE SYSTEMIC INFECTION OF POTATO VIRUS Y 
(PVYO)

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RAKHSHANDEHROU F.; SHAHSAVAN BEHBOUDI BEHROUZ; ZAMANIZADEH H.R.

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Journal: JOURNAL OF SCIENCE (UNIVERSITY OF TEHRAN) (JSUT) Year:2009 | Volume:35 | Issue:1 (SECTION: BIOLOGY) Page(s): 9-16

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Title

TOBACCO ANTIOXIDANT ENZYMES INVOLVEMENT IN RESISTANCE TO THE SYSTEMIC INFECTION OF POTATO VIRUS Y (PVYO)

Author(s)

RAKHSHANDEHROU F. | SHAHSAVAN BEHBOUDI BEHROUZ | ZAMANIZADEH H.R. | Issue Writer Certificate

Keywords

GENE SILENCINGQ3

ANTIOXIDANTS ENZYMESQ3

SUSCEPTIBILITYQ3

RDR1Q3

POTATO VIRUS YQ3

Abstract

RNA-directed RNA polymerases are main elements of the post transcriptional GENE SILENCING (PTGS) pathway within eukaryotic cells. Previous studies indicated to the effective role of salicylic acid in PTGS phenomenon by inducing RDR1 gene expression. Using deifferent lines of transgenic tobacco plants in which the RDR1 gene was silenced moderately or highly in compare to the wildtype we evaluate the role of RDR1in tobacco (N. tabacum cv Samsun, NN) resistance to the systemic infection of POTATO VIRUS Y (PVYO).Antioxidative system of tobacco leaf apoplast including Polyphenoloxidase (PPO) and Peroxidase (POX) were quantitatively and qualitatively assessed for evaluating the resistance levels to POTATO VIRUS Y (PVYO) systemic infection in transgenic assay lines. Using semi-quantitative RTPCR method tobacco RDR1 and PVYO coat protein genes expression level were quantitatively determined through the RDR1 transgenic silenced tobacco lines and wildtype. Results of this study showed that, highly RDR1 silenced transgenic line accumulate the coat protein gene of PVY more than the wild type and moderately RDR1 silenced tobacco plant. In compare to the transgenic lines, considerable increase in polyphenoloxidase (PPO) specific activity observed for non transgenic tobacco wild type up to one month after PVY inoculation.However Peroxidase (POX) specific activity was higher in RDR1 silenced transgenic lines. Higher SUSCEPTIBILITY to PVY infection through the RDR1 silenced transgenic lines was concurrent with the higher specific activity of Peroxidase enzyme. Our data elucidate the effective role of RDR1 gene in resistance of tobacco plants to systemic viral infection. Additionally enzymatic changes related to the resistance response against systemic viral infections proved for the first time.

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APA: Copy

RAKHSHANDEHROU, F., SHAHSAVAN BEHBOUDI, BEHROUZ, & ZAMANIZADEH, H.R.. (2009). TOBACCO ANTIOXIDANT ENZYMES INVOLVEMENT IN RESISTANCE TO THE SYSTEMIC INFECTION OF POTATO VIRUS Y (PVY^O). JOURNAL OF SCIENCE (UNIVERSITY OF TEHRAN) (JSUT), 35(1 (SECTION: BIOLOGY)), 9-16. SID. https://sid.ir/paper/2174/en

Vancouver: Copy

RAKHSHANDEHROU F., SHAHSAVAN BEHBOUDI BEHROUZ, ZAMANIZADEH H.R.. TOBACCO ANTIOXIDANT ENZYMES INVOLVEMENT IN RESISTANCE TO THE SYSTEMIC INFECTION OF POTATO VIRUS Y (PVY^O). JOURNAL OF SCIENCE (UNIVERSITY OF TEHRAN) (JSUT)[Internet]. 2009;35(1 (SECTION: BIOLOGY)):9-16. Available from: https://sid.ir/paper/2174/en

IEEE: Copy

F. RAKHSHANDEHROU, BEHROUZ SHAHSAVAN BEHBOUDI, and H.R. ZAMANIZADEH, “TOBACCO ANTIOXIDANT ENZYMES INVOLVEMENT IN RESISTANCE TO THE SYSTEMIC INFECTION OF POTATO VIRUS Y (PVY^O),” JOURNAL OF SCIENCE (UNIVERSITY OF TEHRAN) (JSUT), vol. 35, no. 1 (SECTION: BIOLOGY), pp. 9–16, 2009, [Online]. Available: https://sid.ir/paper/2174/en

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