C-, G-, NOR- AND RESTRICTION ENZYME- BANDING AND DAPI STAINING OF CHROMOSOMES OF ZAGROS PUPFISH APHANIUS VLADYKOVI

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HEMAT ZADE A.; AMINI F.

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Paper Information

Journal: JOURNAL OF AQUATIC ANIMALS & FISHERIES Year:2012 | Volume:3 | Issue:11 Page(s): 59-66

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Title

C-, G-, NOR- AND RESTRICTION ENZYME- BANDING AND DAPI STAINING OF CHROMOSOMES OF ZAGROS PUPFISH APHANIUS VLADYKOVI

Author(s)

HEMAT ZADE A. | AMINI F. | Issue Writer Certificate

Keywords

CHROMOSOM BANDINGSQ2

ZAGROS PUPFISHQ2

APHANIUS VLADYKOVIQ2

CHAHARMAHAL- VA- BAKHTIARI PROVINCEQ2

IRANQ2

Abstract

Zagros pupfish (Aphanius vladykovi) is a native species of IRAN living in Zagros mountains (in CHAHARMAHAL- VA- BAKHTIARI PROVINCE). Because of the close habitats, this species is important in terms of biodiversity and evolution studies. several methods of staining and banding protocols including DAPI staining as well as C-, G-, NOR- and restriction enzyme- banding were used in this study. Diploid chromosome number for this species was found with modal number of 2n=48. G- banding produced distinguishable bands, however, C-banding did not show any clear bands suggesting lack of protein rich regions. Ag – NOR banding resulted in several bands particularly in telomeric regions of the chromosomes. In RE- banding experiments Alu I and BamH I restriction enzymes produced clear bands whereas Pst I failed to produce any clear bands.

Cites

No record.

References

CHROMOSOMAL STUDY ON ZAGROS PUPFISH (APHANIUS VLADYKOVI)

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APA: Copy

HEMAT ZADE, A., & AMINI, F.. (2012). C-, G-, NOR- AND RESTRICTION ENZYME- BANDING AND DAPI STAINING OF CHROMOSOMES OF ZAGROS PUPFISH APHANIUS VLADYKOVI. JOURNAL OF AQUATIC ANIMALS & FISHERIES, 3(11), 59-66. SID. https://sid.ir/paper/219834/en

Vancouver: Copy

HEMAT ZADE A., AMINI F.. C-, G-, NOR- AND RESTRICTION ENZYME- BANDING AND DAPI STAINING OF CHROMOSOMES OF ZAGROS PUPFISH APHANIUS VLADYKOVI. JOURNAL OF AQUATIC ANIMALS & FISHERIES[Internet]. 2012;3(11):59-66. Available from: https://sid.ir/paper/219834/en

IEEE: Copy

A. HEMAT ZADE, and F. AMINI, “C-, G-, NOR- AND RESTRICTION ENZYME- BANDING AND DAPI STAINING OF CHROMOSOMES OF ZAGROS PUPFISH APHANIUS VLADYKOVI,” JOURNAL OF AQUATIC ANIMALS & FISHERIES, vol. 3, no. 11, pp. 59–66, 2012, [Online]. Available: https://sid.ir/paper/219834/en

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