A Hepatic Differentiation Matrix Establishment for Improving the Attachment and Proliferation of Mesenchymal Stem Cells

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Khajeh Sahar; SAHEBGHADAM LOTFI ABBAS; Razban Vahid; MOHSENIFAR AFSHIN; SOLEIMANI MASOUD

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Journal: JOURNAL OF BIOTECHNOLOGY Year:2013 | Volume:4 | Issue:1 Page(s): 1-14

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Title

A Hepatic Differentiation Matrix Establishment for Improving the Attachment and Proliferation of Mesenchymal Stem Cells

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Keywords

Mesenchymal stem cellsQ2

Hepatic differentiationQ2

Cell attachmentQ2

GlycoseaminoglycanQ2

Abstract

Most patients with liver diseases are in the waiting list of liver implantation for a long period of time because of the lack of enough donors. Liver differentiation potential of Mesenchymal stem cells (MSCs) is a new perspective in curing these patients. Tissue engineering improves hepatocyte differentiation by coating the culture surfaces with Glycosaminoglycans (GAGs) such as Heparan Sulfate (HS). Cell detachment and death during hepatogenic differentiation hamper the efficiency of cell therapy. This study aims to establish a matrix. mimicking the liver extracellular matrix, which supports the attachment and proliferation potential of MSCs, as well. Collagen was physically coated on polystyrene plates. Collagen-GAG matrix was constructed by covalently immoblizing the HS molecules on collagen by EDC. Cell attachment and proliferation were evaluated by direct cell-counting and MTT methods. GAG presence on collagen was verified by Safranin O staining. Comparisons showed that the highest attachment belonged to collagen, collagen-HS and polystyrene, respectively. Collagen matrix showed also the highest cell proliferation. Collagen-GAG provided more suitable matrix for cell proliferation compared to polystyrene. The results further showed that biomimicked collagen-GAG matrix supports superior attachment and viability for MSCs compared to polystyrene.

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APA: Copy

Khajeh, Sahar, SAHEBGHADAM LOTFI, ABBAS, Razban, Vahid, MOHSENIFAR, AFSHIN, & SOLEIMANI, MASOUD. (2013). A Hepatic Differentiation Matrix Establishment for Improving the Attachment and Proliferation of Mesenchymal Stem Cells. JOURNAL OF BIOTECHNOLOGY, 4(1 ), 1-14. SID. https://sid.ir/paper/231203/en

Vancouver: Copy

Khajeh Sahar, SAHEBGHADAM LOTFI ABBAS, Razban Vahid, MOHSENIFAR AFSHIN, SOLEIMANI MASOUD. A Hepatic Differentiation Matrix Establishment for Improving the Attachment and Proliferation of Mesenchymal Stem Cells. JOURNAL OF BIOTECHNOLOGY[Internet]. 2013;4(1 ):1-14. Available from: https://sid.ir/paper/231203/en

IEEE: Copy

Sahar Khajeh, ABBAS SAHEBGHADAM LOTFI, Vahid Razban, AFSHIN MOHSENIFAR, and MASOUD SOLEIMANI, “A Hepatic Differentiation Matrix Establishment for Improving the Attachment and Proliferation of Mesenchymal Stem Cells,” JOURNAL OF BIOTECHNOLOGY, vol. 4, no. 1 , pp. 1–14, 2013, [Online]. Available: https://sid.ir/paper/231203/en

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