DIFFERENTIATION OF HUMAN BONE MARROW MESENCHYMAL STEM CELLS TO NEURAL- LIKE CELLS IN VITRO

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

NEMATI SH.; ZARE MEHRJERDI N.; BAHARVAND H.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Tehran University Medical Journal Year:2009 | Volume:67 | Issue:8 Page(s): 527-534

Download Full-Text

Persian Verion

View:

2,433

Download:

Cites:

Information Journal Paper

Title

DIFFERENTIATION OF HUMAN BONE MARROW MESENCHYMAL STEM CELLS TO NEURAL- LIKE CELLS IN VITRO

Author(s)

NEMATI SH. | ZARE MEHRJERDI N. | BAHARVAND H. | Issue Writer Certificate

Keywords

MESENCHYMAL STEM CELLSQ2

NEURAL CELLSQ3

MARKERSQ3

INDUCTIONQ3

DIFFERENTIATIONQ3

Abstract

Background: Human bone marrow MESENCHYMAL STEM CELLS (hMSCs) can differentiate into several types of mesenchymal cells, including osteocytes, chondrocytes, and adipocytes, but can also differentiate into non-mesenchymal cells, such as NEURAL CELLS, under appropriate experimental conditions. Until now, many protocols for inducing neuro-differentiation in MSCs in vitro have been reported. In this study, we induced DIFFERENTIATION into neural phenotype in the hMSCs population by new protocol. In this treatment, hMSCs could express neural MARKERS more than other reports, associating with remarkable morphological modifications.Methods: The Bone marrow specimens were aspirated from the iliac crest of normal men. hMSCs were isolated and cultured in DMEM containing 15% FBS.Between 4-8 passages conversion of hMSCs into neurosphere-like structures and INDUCTION this cells to nerve precursors in the low-attachment plastic bacterial dishes with bFGF, EGF & RA were initiated. After seven days terminal neural DIFFERENTIATION was initiated by plating the cells on poly-L-ornithin and Laminin coated dishes. Cells were differentiated for 7-14 days. We used flowcytometry and immunocytochemistry analysis for assessment of specific neural stem cell MARKERS in induced cells.Results: Flowcytometery analysis showed that after INDUCTION, 90±2.52 percent of the cells will express neuronal marker Nestin and about 41±1 percent of the cells will express Tuj-1 and about 67±1.05 percent of the cells will express GFAP.Immunocytochemistry and morphologically modifications revealed the same results.Conclusion: Results showed that hMSCs treatment with bFGF, EGF& RA the number of Tuj1 neurons. These data confirmed that hMSCs can exhibit neuronal DIFFERENTIATION potential in vitro, depending on the protocols of inducement.

Cites

No record.

References

No record.

Cite

APA: Copy

NEMATI, SH., ZARE MEHRJERDI, N., & BAHARVAND, H.. (2009). DIFFERENTIATION OF HUMAN BONE MARROW MESENCHYMAL STEM CELLS TO NEURAL- LIKE CELLS IN VITRO. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), 67(8), 527-534. SID. https://sid.ir/paper/38454/en

Vancouver: Copy

NEMATI SH., ZARE MEHRJERDI N., BAHARVAND H.. DIFFERENTIATION OF HUMAN BONE MARROW MESENCHYMAL STEM CELLS TO NEURAL- LIKE CELLS IN VITRO. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ)[Internet]. 2009;67(8):527-534. Available from: https://sid.ir/paper/38454/en

IEEE: Copy

SH. NEMATI, N. ZARE MEHRJERDI, and H. BAHARVAND, “DIFFERENTIATION OF HUMAN BONE MARROW MESENCHYMAL STEM CELLS TO NEURAL- LIKE CELLS IN VITRO,” TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), vol. 67, no. 8, pp. 527–534, 2009, [Online]. Available: https://sid.ir/paper/38454/en

Related Journal Papers