Effect of Organic Solvents on the Activity and Stability of Trypsin and Its Stabilization by Sucrose

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

PAZHANG MOHAMMAD; MEHRNEJAD FARAMARZ; CHAPARZADE NADER; CHAPARZADE NADER; RAHMAN POOR RAHMAN POOR

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF BIOTECHNOLOGY Year:2013 | Volume:4 | Issue:1 Page(s): 74-83

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

542

Download:

Cites:

Information Journal Paper

Title

Effect of Organic Solvents on the Activity and Stability of Trypsin and Its Stabilization by Sucrose

Author(s)

Keywords

Enzyme stabilizationQ2

Organic solventQ2

TrypsinQ2

AdditiveQ2

SucroseQ2

Abstract

The use of enzymes in Organic solvents has biotechnological and industrial importance. Organic solvents can decrease the stability of enzymes that is a challenge for the use of enzymes in organic media. There are several approaches such as protein engineering, chemical modification, and use of Additives for stabilization of enzymes in Organic solvents. In this study, activity and stability of Trypsin were investigated in the presence of different Organic solvents. Then the effect of Sucrose on the stability of the enzyme was investigated in the absence and prescence of solvents. The result showed that the activity and stability of Trypsin were decreased in the presence of Organic solvents. DMF had a lowest effect on the activity and stability of the enzyme. The use of Sucrose increased the stability of Trypsin in the presence of Organic solvents. The stabilization effect of Sucrose in the presence of DMF was more than other solvents. Consequently, a mixture of DMF and Sucrose is proposed for the use of Trypsin in industrial applications.

Cites

No record.

References

No record.

Cite

APA: Copy

PAZHANG, MOHAMMAD, MEHRNEJAD, FARAMARZ, CHAPARZADE, NADER, & RAHMAN POOR, RAHMAN POOR. (2013). Effect of Organic Solvents on the Activity and Stability of Trypsin and Its Stabilization by Sucrose. JOURNAL OF BIOTECHNOLOGY, 4(1 ), 74-83. SID. https://sid.ir/paper/231204/en

Vancouver: Copy

PAZHANG MOHAMMAD, MEHRNEJAD FARAMARZ, CHAPARZADE NADER, RAHMAN POOR RAHMAN POOR. Effect of Organic Solvents on the Activity and Stability of Trypsin and Its Stabilization by Sucrose. JOURNAL OF BIOTECHNOLOGY[Internet]. 2013;4(1 ):74-83. Available from: https://sid.ir/paper/231204/en

IEEE: Copy

MOHAMMAD PAZHANG, FARAMARZ MEHRNEJAD, NADER CHAPARZADE, and RAHMAN POOR RAHMAN POOR, “Effect of Organic Solvents on the Activity and Stability of Trypsin and Its Stabilization by Sucrose,” JOURNAL OF BIOTECHNOLOGY, vol. 4, no. 1 , pp. 74–83, 2013, [Online]. Available: https://sid.ir/paper/231204/en

Related Journal Papers