The Effect of Acidic and Amidic Osteocalcin Derived Peptides on the Formation of Nano-hydroxyapatite Crystals

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HOSEINI SAMANE; NADERI MANESH HOSSEIN; FAGHIHI SHAHAB

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Journal Paper

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Journal: JOURNAL OF BIOTECHNOLOGY Year:2013 | Volume:4 | Issue:1 Page(s): 65-74

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Information Journal Paper

Title

The Effect of Acidic and Amidic Osteocalcin Derived Peptides on the Formation of Nano-hydroxyapatite Crystals

Author(s)

HOSEINI SAMANE | NADERI MANESH HOSSEIN | FAGHIHI SHAHAB | Issue Writer Certificate

Keywords

BiomimeticQ2

HydroxyapatiteQ3

CQ2

terminal amidationQ2

BiomineralizationQ2

Abstract

The aim of this study is to investigate the relationship between the structure and function of an osteocalcin derived peptide on Hydroxyapatite nanocrystal formation. For this purpose, , a natural motif sequence consisting of 13 amino acids present in the first helix of osteocalcin was selected based on its calcium binding ability and synthesized in both acidic and amidic forms using solid phase method. Circular dichroism (CD) and electron microscopy were performed to examine the structure and function of synthesized peptides. Moreover, the effect of these peptides on the viability of osteoblast cells was evaluated. Electron microscopy analysis showed the formation of plate-like HA nanocrystals in the presence of amidic peptide. In contrast, amorphous calcium phosphate was formed in the presence of acidic peptide. CD spectra analysis confirmed the random coil structure with lower molar elipticity for amidic peptide. In addition, the amidic peptide significantly increased the proliferation of osteoblast cells. It is concluded that increased bioactivity, which only occurred in amidic peptide is attributable to C-terminal amidation. It is also proposed that peptides with the ability to promote HA formation have the potential to be utilized in hard tissue regeneration high bioactivity and biocompatibility.

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APA: Copy

HOSEINI, SAMANE, NADERI MANESH, HOSSEIN, & FAGHIHI, SHAHAB. (2013). The Effect of Acidic and Amidic Osteocalcin Derived Peptides on the Formation of Nano-hydroxyapatite Crystals. JOURNAL OF BIOTECHNOLOGY, 4(1 ), 65-74. SID. https://sid.ir/paper/231207/en

Vancouver: Copy

HOSEINI SAMANE, NADERI MANESH HOSSEIN, FAGHIHI SHAHAB. The Effect of Acidic and Amidic Osteocalcin Derived Peptides on the Formation of Nano-hydroxyapatite Crystals. JOURNAL OF BIOTECHNOLOGY[Internet]. 2013;4(1 ):65-74. Available from: https://sid.ir/paper/231207/en

IEEE: Copy

SAMANE HOSEINI, HOSSEIN NADERI MANESH, and SHAHAB FAGHIHI, “The Effect of Acidic and Amidic Osteocalcin Derived Peptides on the Formation of Nano-hydroxyapatite Crystals,” JOURNAL OF BIOTECHNOLOGY, vol. 4, no. 1 , pp. 65–74, 2013, [Online]. Available: https://sid.ir/paper/231207/en

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