Transient Expression of Recombinant PARS II Endonuclease Enzyme Using Agroinfiltration in Tobacco

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FARSI M.; MIRZAEI M.; ZOLALA J.

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Journal: JOURNAL OF BIOTECHNOLOGY Year:2018 | Volume:9 | Issue:4 Page(s): 627-633

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Information Journal Paper

Title

Transient Expression of Recombinant PARS II Endonuclease Enzyme Using Agroinfiltration in Tobacco

Author(s)

FARSI M. | MIRZAEI M. | ZOLALA J. | Issue Writer Certificate

Keywords

Single-Strand-Specific NucleasesQ1

PARS II EndonucleaseQ1

AgroinfiltrationQ1

TobaccoQ2

Abstract

Aims The production of recombinant proteins in transgenic plants (molecular farming) is considered a functional aspect of genetic engineering. Unlike animal and bacterial cell-based production systems, proteins produced by plants are very safe and have low production costs due to the absence of common pathogens in humans and animals. The aim of this study was the transient expression of recombinant PARS II Endonuclease enzyme using Agroinfiltration in Tobacco. Materials & Methods In this experimental study, the possibility of producing a recombinant form of PARS II Endonuclease was investigated, using transient expression system via Agrobacterium. The pBI-Pars expression construct (based on the binary vector pBI121) containing the full sequence of the PARS II encoder, upstream kozak, and a downstream 8x-His tag sequence, was infiltrated into Nicotiana tabacum leaves with Agroinfiltration method. After 72 hours, the expression of PARS II gene in agroinfiltrated leave samples was confirmed through Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) and protein Dot-blot, using Anti-His antibody at the levels of mRNA and protein, respectively. Findings The accuracy of the constructed expression construct was confirmed, and the results of Dot-blot by Anti-His antibody confirmed the expression of the recombinant PARS II protein, while no recombinant protein expression was observed in agroinfiltrated control plants with pBI121 construct. Significant amounts of recombinant PARS II nucleases were produced in Tobacco leaves. Conclusion Agroinfiltration is an effective and short-term method for mass production of pure recombinant PARS II nucleases in Tobacco.

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APA: Copy

FARSI, M., MIRZAEI, M., & ZOLALA, J.. (2018). Transient Expression of Recombinant PARS II Endonuclease Enzyme Using Agroinfiltration in Tobacco. JOURNAL OF BIOTECHNOLOGY, 9(4 ), 627-633. SID. https://sid.ir/paper/231308/en

Vancouver: Copy

FARSI M., MIRZAEI M., ZOLALA J.. Transient Expression of Recombinant PARS II Endonuclease Enzyme Using Agroinfiltration in Tobacco. JOURNAL OF BIOTECHNOLOGY[Internet]. 2018;9(4 ):627-633. Available from: https://sid.ir/paper/231308/en

IEEE: Copy

M. FARSI, M. MIRZAEI, and J. ZOLALA, “Transient Expression of Recombinant PARS II Endonuclease Enzyme Using Agroinfiltration in Tobacco,” JOURNAL OF BIOTECHNOLOGY, vol. 9, no. 4 , pp. 627–633, 2018, [Online]. Available: https://sid.ir/paper/231308/en

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