Determination of ATP-Competitive Inhibitor Drug Toxicity in Anaplastic Thyroid Cancer Based on Cell Characteristics and Three-Dimensional Cell Culture

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SAMIMI GH.; HAGHPANAH V.; IRANI SH.; FALLAH P.; AREFIAN E.; SOLEIMANI M.

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Journal: JOURNAL OF BIOTECHNOLOGY Year:2019 | Volume:10 | Issue:3 Page(s): 503-509

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Title

Determination of ATP-Competitive Inhibitor Drug Toxicity in Anaplastic Thyroid Cancer Based on Cell Characteristics and Three-Dimensional Cell Culture

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Keywords

Anaplastic thyroid carcinomaQ1

Cell Culture TechniqueQ1

Drug Screening AssayQ1

Abstract

Aims Three-dimensional (3D) cell culture systems are important because simulating the physiological microenvironment and representing more similarity to “ in vivo” conditions for anticancer drug screening. Taking the advantages of 3D cell culture in the cancer therapy field, we have developed the 3D in vitro anaplastic thyroid cancer (ATC) model for determining the cytotoxic dose of “ BI-847325” chemotherapy agent in ATC cell lines with different genetic background. Materials and Methods C643 and SW1736 ATC cell lines were grown in alginate scaffold. Beads were incubated in medium for one week. Cells were treated with different doses (1-64μ M) of BI-847325 for 24h. The cytotoxic effect of BI-847325 on 3D cultured cell lines was studied by MTT [3-(4, 5-dimethyl thiazolyl-2)-2, 5-diphenyltetrazolium bromide] assay. The survival rate of alginate-encapsulated cells was analyzed by CFSE (5, 6-Carboxyfluorescein N-hydroxysuccinimidyl ester) staining in effective doses for each of the cell lines. Findings Cytotoxic effect of BI-847325 anticancer drug was different for two ATC cell lines. Effective doses of BI-847325 for C643 and SW1736 cell lines were at 25μ M and 43μ M, respectively. CFSE staining analysis confirmed these data. Conclusion Overall, the results of the present study showed that the cytotoxic effect of BI-847325 chemotherapy agent was different for two ATC cell lines. The importance of this subject in regard to the 3D cell culture methods can be useful for researchers in the design of the complementary experience in order to achieve the most appropriate chemotherapy drug with the most effective dose.

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APA: Copy

SAMIMI, GH., HAGHPANAH, V., IRANI, SH., FALLAH, P., AREFIAN, E., & SOLEIMANI, M.. (2019). Determination of ATP-Competitive Inhibitor Drug Toxicity in Anaplastic Thyroid Cancer Based on Cell Characteristics and Three-Dimensional Cell Culture. JOURNAL OF BIOTECHNOLOGY, 10(3 ), 503-509. SID. https://sid.ir/paper/231352/en

Vancouver: Copy

SAMIMI GH., HAGHPANAH V., IRANI SH., FALLAH P., AREFIAN E., SOLEIMANI M.. Determination of ATP-Competitive Inhibitor Drug Toxicity in Anaplastic Thyroid Cancer Based on Cell Characteristics and Three-Dimensional Cell Culture. JOURNAL OF BIOTECHNOLOGY[Internet]. 2019;10(3 ):503-509. Available from: https://sid.ir/paper/231352/en

IEEE: Copy

GH. SAMIMI, V. HAGHPANAH, SH. IRANI, P. FALLAH, E. AREFIAN, and M. SOLEIMANI, “Determination of ATP-Competitive Inhibitor Drug Toxicity in Anaplastic Thyroid Cancer Based on Cell Characteristics and Three-Dimensional Cell Culture,” JOURNAL OF BIOTECHNOLOGY, vol. 10, no. 3 , pp. 503–509, 2019, [Online]. Available: https://sid.ir/paper/231352/en

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