COMPARISON OF THREE DIFFERENT DNA EXTRACTION METHODS FROM DIFFERENT TISSUE SAMPLES OF THE MARINE CONE SNAIL, CONUS CORONATUS

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RAJABI HALIMEH; ZOLGHARNEN HOSSEIN; RONAGH MOHAMMAD TAGHI; SAVARI AHMAD; RANJBAR MOHAMMAD SHARIF

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Journal: EXPERIMENTAL ANIMAL BIOLOGY Year:2016 | Volume:4 | Issue:4 (16) Page(s): 19-25

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Title

COMPARISON OF THREE DIFFERENT DNA EXTRACTION METHODS FROM DIFFERENT TISSUE SAMPLES OF THE MARINE CONE SNAIL, CONUS CORONATUS

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Keywords

CONUS CORONATUSQ1

DNA EXTRACTIONQ2

MOLLUSCAQ2

SPECTROPHOTOMETERQ1

Abstract

Genomic DNA EXTRACTION with high quantity and quality is the basic necessities of molecular genetic studies. The aim of this study is the applicability of different DNA EXTRACTION methods as the easiest and fastest methods for and their efficiency with different tissue qualities (foot, siphon, harpoon) from marine cone snailCONUS CORONATUS, MOLLUSCA with Phenol-chloroform, Ammonium acetate and CTAB (Cetyl trimethylammonium bromide) methods. About 30 samples were collected from Qeshm Island, Zeyton Park. After dissection on sterile condition, different kinds of Conus tissues were separated and preserved in ethanol 96%, then transferred to the lab. DNA EXTRACTION were performed from different tissues with different methods. SPECTROPHOTOMETER and electrophoresis on 1% agarose gel were used for evaluating the quality and quantity of extracted DNA samples. According to the results, Ammonium acetate method showed sharper bands, without esmire in comparison with other extraction methods and DNA extracted from foot tissue had the best quality and quantity.

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APA: Copy

RAJABI, HALIMEH, ZOLGHARNEN, HOSSEIN, RONAGH, MOHAMMAD TAGHI, SAVARI, AHMAD, & RANJBAR, MOHAMMAD SHARIF. (2016). COMPARISON OF THREE DIFFERENT DNA EXTRACTION METHODS FROM DIFFERENT TISSUE SAMPLES OF THE MARINE CONE SNAIL, CONUS CORONATUS. EXPERIMENTAL ANIMAL BIOLOGY, 4(4 (16)), 19-25. SID. https://sid.ir/paper/236074/en

Vancouver: Copy

RAJABI HALIMEH, ZOLGHARNEN HOSSEIN, RONAGH MOHAMMAD TAGHI, SAVARI AHMAD, RANJBAR MOHAMMAD SHARIF. COMPARISON OF THREE DIFFERENT DNA EXTRACTION METHODS FROM DIFFERENT TISSUE SAMPLES OF THE MARINE CONE SNAIL, CONUS CORONATUS. EXPERIMENTAL ANIMAL BIOLOGY[Internet]. 2016;4(4 (16)):19-25. Available from: https://sid.ir/paper/236074/en

IEEE: Copy

HALIMEH RAJABI, HOSSEIN ZOLGHARNEN, MOHAMMAD TAGHI RONAGH, AHMAD SAVARI, and MOHAMMAD SHARIF RANJBAR, “COMPARISON OF THREE DIFFERENT DNA EXTRACTION METHODS FROM DIFFERENT TISSUE SAMPLES OF THE MARINE CONE SNAIL, CONUS CORONATUS,” EXPERIMENTAL ANIMAL BIOLOGY, vol. 4, no. 4 (16), pp. 19–25, 2016, [Online]. Available: https://sid.ir/paper/236074/en

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