ISOLATION AND OPTIMIZATION OF PECTINASE ENZYME PRODUCTION ONE OF USEFUL INDUSTRIAL ENZYME IN ASPERGILLUS NIGER, RHIZOPUS ORYZAE, PENICILIUM CHRYSOGENUM

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SONGOL AKRAM; BEHBAHANI MANDANA

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: BIOLOGICAL JOURNAL OF MICROORGANISM Year:2016 | Volume:5 | Issue:17 Page(s): 121-140

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

2,865

Download:

Cites:

Information Journal Paper

Title

ISOLATION AND OPTIMIZATION OF PECTINASE ENZYME PRODUCTION ONE OF USEFUL INDUSTRIAL ENZYME IN ASPERGILLUS NIGER, RHIZOPUS ORYZAE, PENICILIUM CHRYSOGENUM

Author(s)

SONGOL AKRAM | BEHBAHANI MANDANA | Issue Writer Certificate

Keywords

PECTINASE ENZYMEQ2

ASPERGILLUS FUMIGATUSQ1

RHIZOPUS ORYZAEQ1

PENICILIUM CHRYSOGENUMQ1

OPTIMIZATIONQ2

FACTORIAL DESIGN METHODQ1

Abstract

Introduction: PECTINASE ENZYME is one of the most important industrial enzymes which isolated from a wide variety of microorganisms such as bacteria and filamentous fungi. This enzyme has been usually used in the fruit and textile industry. In this study, the isolation and OPTIMIZATION of pectinase-producing fungi on decaying rotten fruits were studied.Materials and methods: Isolation and screening of pectinase producing fungi performed through plate culture on pectin medium and staining with Lugol's iodine solution. The best strains were identified by ITS1, 4 sequencing as ASPERGILLUS FUMIGATUS, RHIZOPUS ORYZAE, PENICILIUM CHRYSOGENUM. The enzyme production was optimized by application of the five factorial design, each at three levels. These factors are carbon sources (whey, glucose and stevia), ammonium sulfate, manganese sulfate, temperature, and pH. Pectinase concentration was measured by the Miller method.Results: The results indicate that optimum condition for enzyme production for three fungi strains was obtained at 32°C, pH=6, 3 g/L manganese sulfate, 2.75 g/L of ammonium sulfate and 10 g/L of each carbon source. The best experiment in obtaining the optimum enzyme contained 1.328 mg/ml of glucose for Aspergillus niger 1.284 and 1.039 mg/ml of whey for RHIZOPUS ORYZAE and PENICILIUM CHRYSOGENUM. Molecular weight of enzyme was about 40 and 37 kDa which was obtained by SDS- PAGE.Discussion and conclusion: The results indicate that three strains could grow in a wide range of carbon source, pH and temperature, which could be a good candidate for industrial application.

Cites

No record.

References

No record.

Cite

APA: Copy

SONGOL, AKRAM, & BEHBAHANI, MANDANA. (2016). ISOLATION AND OPTIMIZATION OF PECTINASE ENZYME PRODUCTION ONE OF USEFUL INDUSTRIAL ENZYME IN ASPERGILLUS NIGER, RHIZOPUS ORYZAE, PENICILIUM CHRYSOGENUM. BIOLOGICAL JOURNAL OF MICROORGANISM, 5(17), 121-140. SID. https://sid.ir/paper/237310/en

Vancouver: Copy

SONGOL AKRAM, BEHBAHANI MANDANA. ISOLATION AND OPTIMIZATION OF PECTINASE ENZYME PRODUCTION ONE OF USEFUL INDUSTRIAL ENZYME IN ASPERGILLUS NIGER, RHIZOPUS ORYZAE, PENICILIUM CHRYSOGENUM. BIOLOGICAL JOURNAL OF MICROORGANISM[Internet]. 2016;5(17):121-140. Available from: https://sid.ir/paper/237310/en

IEEE: Copy

AKRAM SONGOL, and MANDANA BEHBAHANI, “ISOLATION AND OPTIMIZATION OF PECTINASE ENZYME PRODUCTION ONE OF USEFUL INDUSTRIAL ENZYME IN ASPERGILLUS NIGER, RHIZOPUS ORYZAE, PENICILIUM CHRYSOGENUM,” BIOLOGICAL JOURNAL OF MICROORGANISM, vol. 5, no. 17, pp. 121–140, 2016, [Online]. Available: https://sid.ir/paper/237310/en

Related Journal Papers