ISOLATION AND MOLECULAR IDENTIFICATION OF A NEW STRAIN OF MICROBACTERIUM RESISTENS, CAPABLE OF TOLERATING THE EXTREME CONDITIONS

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GHOLAMI MAHMOUD; ETEMADIFAR ZAHRA

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Journal: BIOLOGICAL JOURNAL OF MICROORGANISM Year:2013 | Volume:2 | Issue:5 Page(s): 27-47

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Title

ISOLATION AND MOLECULAR IDENTIFICATION OF A NEW STRAIN OF MICROBACTERIUM RESISTENS, CAPABLE OF TOLERATING THE EXTREME CONDITIONS

Author(s)

GHOLAMI MAHMOUD | ETEMADIFAR ZAHRA | Issue Writer Certificate

Keywords

UV-RESISTANCEQ2

MICROBACTERIOUM RESISTENSQ2

FLOW CYTOMETRYQ2

EXTREME CONDITIONSQ2

Abstract

Introduction: The use of resistant bacteria to radiation and other EXTREME CONDITIONS for bioremediation of the environmental and radioactive waste is very important due to their survivability and catalytic efficiency under high stress conditions.Materials and methods: After primary and secondary screening by irradiation with UV lamp, one isolate was obtained and identified on the basis of morphological and biochemical characteristics, 16S rRNA gene sequencing and phylogenetic analysis. In the next step, the isolate was exposed to EXTREME CONDITIONS such as: UV-radiation (25J/cm2), desiccation (28 days), %1-5 hydrogen peroxide, mitomycin C (1-8mg), salinity (%5-25 NaCl) and different pHs (pH 1.0-11) during or after culture in TGY medium. Bacterial growth or survival was analyzed by spectrometry or FLOW CYTOMETRY.Results: Isolated strain MG6 was an ultraviolet-resistant, Gram-positive, and rod shaped bacterium that its 16S rDNA sequences showed %99.69 similarity with Microbacterium resistens. This similarity was confirmed by phylogenetic analysis. Optimal growth rate of strain MG6 occurred at pH 5, %5 (w/v) NaCl and tolerated %1- 4 H2O2 and 2mg of mitomycin C. Analysis of viability indicated that this strain had highly resistance to UV-C radiation but moderately resistance to showed desiccation.Discussion and conclusion: In this study, a new strain of M. resistens was isolated which was UV-resistant and showed tolerance to several EXTREME CONDITIONS such as: low pH, oxidant and mutagen agents, salinity and desiccation. The UV resistance of strain MG6 was comparable with Deinococcus radiodurans R1. This research was the first study on multiple extreme resistance of M. resistens new isolated strain (MG6) in Iran. Also this strain could be a candidate for the future researches such as bioremediation of radioactive waste sites.

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APA: Copy

GHOLAMI, MAHMOUD, & ETEMADIFAR, ZAHRA. (2013). ISOLATION AND MOLECULAR IDENTIFICATION OF A NEW STRAIN OF MICROBACTERIUM RESISTENS, CAPABLE OF TOLERATING THE EXTREME CONDITIONS. BIOLOGICAL JOURNAL OF MICROORGANISM, 2(5), 27-47. SID. https://sid.ir/paper/237470/en

Vancouver: Copy

GHOLAMI MAHMOUD, ETEMADIFAR ZAHRA. ISOLATION AND MOLECULAR IDENTIFICATION OF A NEW STRAIN OF MICROBACTERIUM RESISTENS, CAPABLE OF TOLERATING THE EXTREME CONDITIONS. BIOLOGICAL JOURNAL OF MICROORGANISM[Internet]. 2013;2(5):27-47. Available from: https://sid.ir/paper/237470/en

IEEE: Copy

MAHMOUD GHOLAMI, and ZAHRA ETEMADIFAR, “ISOLATION AND MOLECULAR IDENTIFICATION OF A NEW STRAIN OF MICROBACTERIUM RESISTENS, CAPABLE OF TOLERATING THE EXTREME CONDITIONS,” BIOLOGICAL JOURNAL OF MICROORGANISM, vol. 2, no. 5, pp. 27–47, 2013, [Online]. Available: https://sid.ir/paper/237470/en

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