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Information Journal Paper

Title

EFFECTS OF METABOLITES OF LACTOBACILLI ISOLATED FROM LOCAL YOGHURTS IN CHAHRMAHAL VA BAKHTIARY ON PATHOGENIC BACTERIA

Pages

  11-20

Abstract

 Introduction: PROBIOTICS have some beneficial effects on health so these microorganisms are added to dairy products and other foods as well. Lactobacillus bacteria are the most commonly used for this purpose. The aim of this study was to find native Lactobacillus bacteria which has the potentiality for using as probiotic.Materials and methods: For isolating LACTOBACILLI, gram strain reaction and catalase test were performed on separated bacteria from 40 local yoghurt samples: after isolating, the acid resistance ability of isolates were evaluated by placing isolates in pH=2.5 for 2 hours and culturing samples on medium and colony counts. Then, among 18 acid resistant bacteria, by GEL DIFFUSION METHOD the inhibitory effect of isolates on pathogens bacteria such as E.coli and Pseudomonas aeruginosa were investigated. The results were compared to the results of investigated commercial strains.Results: The results revealed that S4 isolate had the most inhibitory effect, it showed %80 good-inhibitory and %20 strong-inhibitory effect on pathogenic bacteria, which were completely consistent with results for commercial Lactobacillus gasseri strain. Also, the lowest inhibitory effect against pathogens was related to (Y4-Y5-Y11-Y14-Y15) isolates that all of them had weak-inhibitory effect on pathogens. Other strains were classified in good-inhibitor group.Discussion and conclusion: Considering daily increasing of drug resistance relating to pathogenic bacteria, today, the use of PROBIOTICS, especially LACTOBACILLI as an appropriate alternative of antibiotics is important. The investigated bacteria in this study were able to inhibit pathogens’s growth to some extent, but for investigating the results more accurately, some methods with more sensitivity, for instance, two layers method and MIC method can be used as well.

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    APA: Copy

    EBRAHIMI ALAVIJEH, SAMIRA, MAHZOUNIEH, MOHAMMADREZA, & MOBINI DEHKORDI, MOHSEN. (2013). EFFECTS OF METABOLITES OF LACTOBACILLI ISOLATED FROM LOCAL YOGHURTS IN CHAHRMAHAL VA BAKHTIARY ON PATHOGENIC BACTERIA. BIOLOGICAL JOURNAL OF MICROORGANISM, 2(5), 11-20. SID. https://sid.ir/paper/237473/en

    Vancouver: Copy

    EBRAHIMI ALAVIJEH SAMIRA, MAHZOUNIEH MOHAMMADREZA, MOBINI DEHKORDI MOHSEN. EFFECTS OF METABOLITES OF LACTOBACILLI ISOLATED FROM LOCAL YOGHURTS IN CHAHRMAHAL VA BAKHTIARY ON PATHOGENIC BACTERIA. BIOLOGICAL JOURNAL OF MICROORGANISM[Internet]. 2013;2(5):11-20. Available from: https://sid.ir/paper/237473/en

    IEEE: Copy

    SAMIRA EBRAHIMI ALAVIJEH, MOHAMMADREZA MAHZOUNIEH, and MOHSEN MOBINI DEHKORDI, “EFFECTS OF METABOLITES OF LACTOBACILLI ISOLATED FROM LOCAL YOGHURTS IN CHAHRMAHAL VA BAKHTIARY ON PATHOGENIC BACTERIA,” BIOLOGICAL JOURNAL OF MICROORGANISM, vol. 2, no. 5, pp. 11–20, 2013, [Online]. Available: https://sid.ir/paper/237473/en

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