PRODUCTION OF BIODIESEL FUEL FROM OLIVE OIL AND METHANOL USING NATIVE PSEUDOMONAS SP. LIPASE

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ALIJANYPOOR K.; GHAEMI N.; AMIRMOZAFARI N.; DASTAN K.

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Paper Information

Journal: Biotechnological Journal of Environmental Microorganisms Year:2012 | Volume: | Issue: Page(s): 33-43

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Title

PRODUCTION OF BIODIESEL FUEL FROM OLIVE OIL AND METHANOL USING NATIVE PSEUDOMONAS SP. LIPASE

Author(s)

ALIJANYPOOR K. | GHAEMI N. | AMIRMOZAFARI N. | DASTAN K. | Issue Writer Certificate

Keywords

BOIDIESELQ2

TRANSESTERIFICATIONQ2

OLIVE OILQ2

METHANOLQ2

LIPASEQ2

Abstract

The limited resources and fast diminishing of fossil fuels increasing prices of crude oil, and environmental concerns have been the diverse reasons for exploring the use of vegetable oils as alternative fuel. The biodiesel can be obtained from vegetable oils by TRANSESTERIFICATION with METHANOL / ethanol. The TRANSESTERIFICATION can be carried out chemically or enzymetically. TRANSESTERIFICATION reaction was performed using OLIVE OIL as cheap alternative for triglyceride and short-chain alcohol (methanol) by native Pseudomonas sp. free LIPASE. The long- chain fatty acid ester, which is the product of this reaction, is named biodiesel, can be used as a diesel fuel that does not produce sulfur oxide and minimize the soot particulate. LIPASE that was extracted from native strain of Peudomonas. sp was use for TRANSESTERIFICATION. This strain with most enzyme production was isolated from different soil samples of Guilan province. LIPASE was produced by growing the culture in GYP medium, for 8h at 37oC. Activity of secreted LIPASE was induced by OLIVE OIL. The life of the free enzyme was more than 2 days. For BOIDIESEL production, OLIVE OIL and METHANOL were taken in the ratio of 1:4 (mol/mol-1). To the mixture 2ml of enzyme solution was added and then incubated at 40oC with constant shaking at 200 rpm, for 12h. Formation of methyl ester was analyzed by column chromatography. That final yield of BOIDIESEL was more than %70.

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APA: Copy

ALIJANYPOOR, K., GHAEMI, N., AMIRMOZAFARI, N., & DASTAN, K.. (2012). PRODUCTION OF BIODIESEL FUEL FROM OLIVE OIL AND METHANOL USING NATIVE PSEUDOMONAS SP. LIPASE. BIOTECHNOLOGICAL JOURNAL OF ENVIRONMENTAL MICROORGANISMS, 6(1), 33-43. SID. https://sid.ir/paper/242211/en

Vancouver: Copy

ALIJANYPOOR K., GHAEMI N., AMIRMOZAFARI N., DASTAN K.. PRODUCTION OF BIODIESEL FUEL FROM OLIVE OIL AND METHANOL USING NATIVE PSEUDOMONAS SP. LIPASE. BIOTECHNOLOGICAL JOURNAL OF ENVIRONMENTAL MICROORGANISMS[Internet]. 2012;6(1):33-43. Available from: https://sid.ir/paper/242211/en

IEEE: Copy

K. ALIJANYPOOR, N. GHAEMI, N. AMIRMOZAFARI, and K. DASTAN, “PRODUCTION OF BIODIESEL FUEL FROM OLIVE OIL AND METHANOL USING NATIVE PSEUDOMONAS SP. LIPASE,” BIOTECHNOLOGICAL JOURNAL OF ENVIRONMENTAL MICROORGANISMS, vol. 6, no. 1, pp. 33–43, 2012, [Online]. Available: https://sid.ir/paper/242211/en

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