THE USE OF BETAINE ALDEHYDE DEHYDROGENASE AS A NON-ANTIBIOTIC AND SAFE SELECTIVE MARKER IN DESIGNING OF PLASTID VECTOR

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MOHSENPOUR M.; BABAIAN JELODAR N.A.; TOHIDFAR M.; MOHSENPOUR M.

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Journal: Biotechnological Journal of Environmental Microorganisms Year:2012 | Volume: | Issue: Page(s): 55-62

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Title

THE USE OF BETAINE ALDEHYDE DEHYDROGENASE AS A NON-ANTIBIOTIC AND SAFE SELECTIVE MARKER IN DESIGNING OF PLASTID VECTOR

Author(s)

MOHSENPOUR M. | BABAIAN JELODAR N.A. | TOHIDFAR M. | MOHSENPOUR M. | Issue Writer Certificate

Keywords

BETAINE ALDEHYDE DEHYDROGENASEQ2

GENETIC ENGINEERINGQ2

NON-ANTIBIOTIC MARKER GENEQ1

PLASTID VECTORQ2

Abstract

In most studies related to plants transformation, the antibiotic resistance genes are used for recovery of transgenic plants, but introducing such products into the food chain may lead to biosafety concerns. In this study, BETAINE ALDEHYDE DEHYDROGENASE (BADH) gene was used as non-antibiotic marker in designing of PLASTID VECTOR. The gene sequences from spinach after introns removal decreased from 6111bp to 1494bp, then the codon optimization was performed based on common codon used in the Plastid Genome and eight of internal restriction enzyme recognition sites in coding region were also removed by changing the nucleotide sequences without changing the amino acids. Increasing of TA percent was also considered in this codon optimization. The final sequence of BADH gene was connected to 5' UTR of T7gene10 and placed under strong Prrn chloroplast promoter by SOEing PCR. RBS and MCS were inserted between promoter and BADH. By cloning of desired genes in this position, expression of them will be polycistronic with BADH and not only Plastid transformation will be possible without antibiotic selective marker, but also we would expect transgenic plants that express BADH, to be resistant to salt, drought and cold stresses.

Cites

DESIGN OF AGROBACTERIUM CONSTRUCTS FOR PRODUCTION OF ANTIBIOTIC FREE TRANSGENIC PLANTS, SALT, DROUGHT AND COLD RESISTANT

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APA: Copy

MOHSENPOUR, M., BABAIAN JELODAR, N.A., TOHIDFAR, M., & MOHSENPOUR, M.. (2012). THE USE OF BETAINE ALDEHYDE DEHYDROGENASE AS A NON-ANTIBIOTIC AND SAFE SELECTIVE MARKER IN DESIGNING OF PLASTID VECTOR. BIOTECHNOLOGICAL JOURNAL OF ENVIRONMENTAL MICROORGANISMS, 6(3 (3)), 55-62. SID. https://sid.ir/paper/242226/en

Vancouver: Copy

MOHSENPOUR M., BABAIAN JELODAR N.A., TOHIDFAR M., MOHSENPOUR M.. THE USE OF BETAINE ALDEHYDE DEHYDROGENASE AS A NON-ANTIBIOTIC AND SAFE SELECTIVE MARKER IN DESIGNING OF PLASTID VECTOR. BIOTECHNOLOGICAL JOURNAL OF ENVIRONMENTAL MICROORGANISMS[Internet]. 2012;6(3 (3)):55-62. Available from: https://sid.ir/paper/242226/en

IEEE: Copy

M. MOHSENPOUR, N.A. BABAIAN JELODAR, M. TOHIDFAR, and M. MOHSENPOUR, “THE USE OF BETAINE ALDEHYDE DEHYDROGENASE AS A NON-ANTIBIOTIC AND SAFE SELECTIVE MARKER IN DESIGNING OF PLASTID VECTOR,” BIOTECHNOLOGICAL JOURNAL OF ENVIRONMENTAL MICROORGANISMS, vol. 6, no. 3 (3), pp. 55–62, 2012, [Online]. Available: https://sid.ir/paper/242226/en

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