DESIGN OF AGROBACTERIUM CONSTRUCTS FOR PRODUCTION OF ANTIBIOTIC FREE TRANSGENIC PLANTS, SALT, DROUGHT AND COLD RESISTANT

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MOHSENPOUR M.; TOHIDFAR M.; MOHSENPOUR MOHADESE

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Journal: Biotechnological Journal of Environmental Microorganisms Year:2012 | Volume: | Issue: Page(s): 73-80

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Information Journal Paper

Title

DESIGN OF AGROBACTERIUM CONSTRUCTS FOR PRODUCTION OF ANTIBIOTIC FREE TRANSGENIC PLANTS, SALT, DROUGHT AND COLD RESISTANT

Author(s)

MOHSENPOUR M. | TOHIDFAR M. | MOHSENPOUR M.HADESE | Issue Writer Certificate

Keywords

AGROBACTERIUM VECTORQ2

BETAINE ALDEHYDE DEHYDROGENASEQ2

NON-ANTIBIOTIC MARKER GENEQ2

Abstract

In most studies related to plants transformation, the antibiotic resistance genes are used for recovery of transgenic plants, but introducing such products into the food chain may lead to biosafety concerns. In this study, BETAINE ALDEHYDE DEHYDROGENASE gene (BADH) was used as a safe and valuable non-antibiotic marker for construction of AGROBACTERIUM VECTORs suitable for plant transformation. This gene sequences from spinach decreased from 6111bp to 1494bp after splicing. Nucleotide sequences were changed for remove of eight internal restriction enzyme recognition sites that do not result in a change to the amino acid sequence of BADH protein. badh gene with 5’UTR of T7gene10 was cloned from pB plastid vector into pTZ57R/T vector. This cloning procedure was performed in several stages including enzymatic double digestion, sticky ends to blunt ends conversion, adding of ATG start codon and adding of A nucleotide in the ends of fragment. Then this fragment was Re-cloned from pTZ-BADH recombinant plasmid into T-DNA regions of two Agrobacterium binary vectors. Finally in this study two recombinant vectors were obtained, pBI-BADH and pBI (-k) BADH, that containing badh gene under control of CaMV35S promoter and Nos terminator. pBI (-k) BADH vector is an antibiotic marker free vector that can be used for plant transformation in order to producing resistant plants against salt, drought and cold stresses.

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References

THE USE OF BETAINE ALDEHYDE DEHYDROGENASE AS A NON-ANTIBIOTIC AND SAFE SELECTIVE MARKER IN DESIGNING OF PLASTID VECTOR

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APA: Copy

MOHSENPOUR, M., TOHIDFAR, M., & MOHSENPOUR, MOHADESE. (2012). DESIGN OF AGROBACTERIUM CONSTRUCTS FOR PRODUCTION OF ANTIBIOTIC FREE TRANSGENIC PLANTS, SALT, DROUGHT AND COLD RESISTANT. BIOTECHNOLOGICAL JOURNAL OF ENVIRONMENTAL MICROORGANISMS, 6(1), 73-80. SID. https://sid.ir/paper/242207/en

Vancouver: Copy

MOHSENPOUR M., TOHIDFAR M., MOHSENPOUR MOHADESE. DESIGN OF AGROBACTERIUM CONSTRUCTS FOR PRODUCTION OF ANTIBIOTIC FREE TRANSGENIC PLANTS, SALT, DROUGHT AND COLD RESISTANT. BIOTECHNOLOGICAL JOURNAL OF ENVIRONMENTAL MICROORGANISMS[Internet]. 2012;6(1):73-80. Available from: https://sid.ir/paper/242207/en

IEEE: Copy

M. MOHSENPOUR, M. TOHIDFAR, and MOHADESE MOHSENPOUR, “DESIGN OF AGROBACTERIUM CONSTRUCTS FOR PRODUCTION OF ANTIBIOTIC FREE TRANSGENIC PLANTS, SALT, DROUGHT AND COLD RESISTANT,” BIOTECHNOLOGICAL JOURNAL OF ENVIRONMENTAL MICROORGANISMS, vol. 6, no. 1, pp. 73–80, 2012, [Online]. Available: https://sid.ir/paper/242207/en

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