EVALUATION OF FLAVONOIDS AND PHENOLIC COMPOUNDS, ANTIOXIDANT AND ANTIBACTERIAL ACTIVITY OF HYPERICUM PERFORATUM L. COLLECTED FROM TWO SITES IN NORTH COUNTRY

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KOOHSARI HADI; KHORMALI HAMIDE; KHORMALI AYESHEH

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Journal: Ecophytochemistry journal of Medicinal Plants Year:2017 | Volume:5 | Issue:1 (17) Page(s): 78-90

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Title

EVALUATION OF FLAVONOIDS AND PHENOLIC COMPOUNDS, ANTIOXIDANT AND ANTIBACTERIAL ACTIVITY OF HYPERICUM PERFORATUM L. COLLECTED FROM TWO SITES IN NORTH COUNTRY

Author(s)

KOOHSARI HADI | KHORMALI HAMIDE | KHORMALI AYESHEH | Issue Writer Certificate

Keywords

ANTIOXIDANTQ1

ANTIBACTERIALQ1

HYPERICUM PERFORATUM LQ2

MAZANDARAN PROVINCEQ1

TOTAL FLAVONOIDS AND PHENOLICQ2

Abstract

This study was carried out to evaluate the phytochemical, ANTIOXIDANT and ANTIBACTERIAL activity of HYPERICUM PERFORATUM L., the aerial parts of plant were collected from two sites in MAZANDARAN PROVINCE (Farahabad Sari and Pashakola Amol) during July to October 2015. Total phenol and flavonoid contents were determined by colorimetric method using the Folin-Ciocalteu reagent and aluminum chloride respectively. The ANTIOXIDANT activity was measured by DPPH method and then the ANTIBACTERIAL activity was performed by agar well diffusion, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of plant extract were determined by broth micro dilution tube method. The results were showed that the total phenolic and flavonoid content and then the ANTIOXIDANT capacity of plant from Sari region was more than in Amol region. The MIC values which obtained fromSari against Staphylococcus aureus, Bacillus cereus, Shigella dysentery and E. coli were 12, 12, 190 and 390 mg/ml respectively, while these values from Amol were 24, 24, 780 and 1560 mg/ml respectively, which results were also confirmed in agar well diffusion method and then in conclusion the plant extract from Sari region had the highest content of secondary metabolites, ANTIOXIDANT and ANTIBACTERIAL activity against tested bacteria and there is the direct correlation between phyto chemistry and their ANTIOXIDANT and ANTIBACTERIAL activity of plant.

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APA: Copy

KOOHSARI, HADI, KHORMALI, HAMIDE, & KHORMALI, AYESHEH. (2017). EVALUATION OF FLAVONOIDS AND PHENOLIC COMPOUNDS, ANTIOXIDANT AND ANTIBACTERIAL ACTIVITY OF HYPERICUM PERFORATUM L. COLLECTED FROM TWO SITES IN NORTH COUNTRY. ECO-PHYTOCHEMICAL JOURNAL OF MEDICAL PLANTS, 5(1 (17)), 78-90. SID. https://sid.ir/paper/247883/en

Vancouver: Copy

KOOHSARI HADI, KHORMALI HAMIDE, KHORMALI AYESHEH. EVALUATION OF FLAVONOIDS AND PHENOLIC COMPOUNDS, ANTIOXIDANT AND ANTIBACTERIAL ACTIVITY OF HYPERICUM PERFORATUM L. COLLECTED FROM TWO SITES IN NORTH COUNTRY. ECO-PHYTOCHEMICAL JOURNAL OF MEDICAL PLANTS[Internet]. 2017;5(1 (17)):78-90. Available from: https://sid.ir/paper/247883/en

IEEE: Copy

HADI KOOHSARI, HAMIDE KHORMALI, and AYESHEH KHORMALI, “EVALUATION OF FLAVONOIDS AND PHENOLIC COMPOUNDS, ANTIOXIDANT AND ANTIBACTERIAL ACTIVITY OF HYPERICUM PERFORATUM L. COLLECTED FROM TWO SITES IN NORTH COUNTRY,” ECO-PHYTOCHEMICAL JOURNAL OF MEDICAL PLANTS, vol. 5, no. 1 (17), pp. 78–90, 2017, [Online]. Available: https://sid.ir/paper/247883/en

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