DETECTING A NOVEL MOLECULAR INTERACTION INVOLVED IN EPIGENETIC CONTROL OF FLC EXPRESSION IN ARABIDOPSIS USING YEAST TWO HYBRID SYSTEM

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

HOSSEINPOUR MARYAM; Pazhouhandeh Maghsoud; MAHMOUDI KURDI FATEMEH

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Molecular and Cellular Researches Year:2016 | Volume:29 | Issue:1 Page(s): 59-71

Download Full-Text

Persian Verion

View:

900

Download:

Cites:

Information Journal Paper

Title

DETECTING A NOVEL MOLECULAR INTERACTION INVOLVED IN EPIGENETIC CONTROL OF FLC EXPRESSION IN ARABIDOPSIS USING YEAST TWO HYBRID SYSTEM

Author(s)

HOSSEINPOUR MARYAM | Pazhouhandeh Maghsoud | MAHMOUDI KURDI FATEMEH | Issue Writer Certificate

Keywords

FLOWERINGQ2

YEAST TWO-HYBRID SYSTEMQ1

MSI4Q1

EPIGENETICSQ2

MOLECULAR INTERACTIONQ1

Abstract

Transition of plant from the vegetative to the reproductive stage is one of the most important developmental processes whose molecular mechanism is not fully understood. Initiation of FLOWERING in plants is controlled by various factors such as photoperiod, cold, hormones and epigenetic effects. A major pathway in this process is the epigenetic control of FLC gene, whose expression inhibits the FLOWERING initiation. Removing acetyl groups from FLC gene histons, by MSI4 protein represses FLC expression, and thus, FLOWERING is initiated. However, the molecular mechanism of this protein is largely unknown. Therefore, studing any MOLECULAR INTERACTION with this protein can be helpful to better understand its mode of action. In this research, we used the Yeast Two Hybrid System (Y2HS) to study protein-protein interactions, to find proteins interacting with MSI4. Therefore, first, we cloned the MSI4 gene in proper Y2HS vector. Then, cDNA bank of Arabidopsis thaliana was screened by MSI4 as bait to prey the interactors of MSI4. The protein trapped by this method was PKT3 (Peroxisomal 3-Ketoacyl-CoA Thiolase 3), which is an acetylcoacyl transferase. The function of this protein is to release acetyl groups and deliver them to other molecules, and therefore, it has significant role in many crucial cell processes. This interaction was confirmed by cloning the complete cDNA of PKT3 as well as its homologue, PKT4. The interaction of MSI4-PKT3 is reported here for the first time and opens new horizons for more studies on MSI4 functional mechanism. This finding can be useful in regulation of FLOWERING time in fruits and crops through genetic engineering of this pathway for example by alteration of PKT4 regulation.

Cites

No record.

References

No record.

Cite

APA: Copy

HOSSEINPOUR, MARYAM, Pazhouhandeh, Maghsoud, & MAHMOUDI KURDI, FATEMEH. (2016). DETECTING A NOVEL MOLECULAR INTERACTION INVOLVED IN EPIGENETIC CONTROL OF FLC EXPRESSION IN ARABIDOPSIS USING YEAST TWO HYBRID SYSTEM. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 29(1 ), 59-71. SID. https://sid.ir/paper/248431/en

Vancouver: Copy

HOSSEINPOUR MARYAM, Pazhouhandeh Maghsoud, MAHMOUDI KURDI FATEMEH. DETECTING A NOVEL MOLECULAR INTERACTION INVOLVED IN EPIGENETIC CONTROL OF FLC EXPRESSION IN ARABIDOPSIS USING YEAST TWO HYBRID SYSTEM. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2016;29(1 ):59-71. Available from: https://sid.ir/paper/248431/en

IEEE: Copy

MARYAM HOSSEINPOUR, Maghsoud Pazhouhandeh, and FATEMEH MAHMOUDI KURDI, “DETECTING A NOVEL MOLECULAR INTERACTION INVOLVED IN EPIGENETIC CONTROL OF FLC EXPRESSION IN ARABIDOPSIS USING YEAST TWO HYBRID SYSTEM,” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 29, no. 1 , pp. 59–71, 2016, [Online]. Available: https://sid.ir/paper/248431/en

Related Journal Papers