THE INSTABILITY OF THE INHIBITION OF THE EXPRESSION OF GENES INVOLVED IN RESISTANCE TO VIRUS INFECTION IN TRANSGENIC PLANTS USING HAIRPIN CONSTRUCTS

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RAKHSHANDEHROO F.; PALUKAITIS P.; SHAMS BAKHSH M.

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Journal: Journal of Molecular and Cellular Researches Year:2014 | Volume:26 | Issue:3 Page(s): 401-415

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Title

THE INSTABILITY OF THE INHIBITION OF THE EXPRESSION OF GENES INVOLVED IN RESISTANCE TO VIRUS INFECTION IN TRANSGENIC PLANTS USING HAIRPIN CONSTRUCTS

Author(s)

RAKHSHANDEHROO F. | PALUKAITIS P. | SHAMS BAKHSH M. | Issue Writer Certificate

Keywords

RNA SILENCINGQ2

STEM-LOOP STRUCTUREQ2

((RDR1))Q2

PVYOQ2

Abstract

Transgenic expression of hairpins can induce RNA SILENCING pathways by means of dsRNA with sequence homology to a plant mRNA. Using transgenic tobacco plants (N. tabacum cv. Samsun NN) in which the (RDR1) gene was silenced to different extents by a hairpin structure homologous to the tobacco (RDR1) gene, we evaluated the stability of gene silencing, by analyzing the (RDR1) gene expression levels and the plant susceptibility to systemic infection by PVYo. The second (T2) and third (T3) generations of (RDR1) silenced transgenic lines were tested by semi-quantitative RT-PCR to evaluate the expression levels of defense-related genes including IVR, ERF5, AOX1, and RDR6, one week after PVYo infection. The results showed that T2 transgenic tobacco lines transcribed the (RDR1) and the other defense-related genes to a lower level than the corresponding T3 transgenic lines. The T2 lines were more susceptible to PVYo infection than the T3 lines and the (RDR1) gene was expressed to the same level in the T3 lines as in non-transformed tobacco. The efficiency of hairpin structure-mediated silencing of the (RDR1) gene decreased through different generations. As the (RDR1)-regulated RDR6 is required for the spreading of silencing throughout the plant, we propose that suppressing (RDR1) leads to inhibition of RDR6, which then leads to loss of silencing by the (RDR1) hairpin transgene after vertical gene transmission.

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APA: Copy

RAKHSHANDEHROO, F., PALUKAITIS, P., & SHAMS BAKHSH, M.. (2014). THE INSTABILITY OF THE INHIBITION OF THE EXPRESSION OF GENES INVOLVED IN RESISTANCE TO VIRUS INFECTION IN TRANSGENIC PLANTS USING HAIRPIN CONSTRUCTS. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 26(3), 401-415. SID. https://sid.ir/paper/248472/en

Vancouver: Copy

RAKHSHANDEHROO F., PALUKAITIS P., SHAMS BAKHSH M.. THE INSTABILITY OF THE INHIBITION OF THE EXPRESSION OF GENES INVOLVED IN RESISTANCE TO VIRUS INFECTION IN TRANSGENIC PLANTS USING HAIRPIN CONSTRUCTS. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2014;26(3):401-415. Available from: https://sid.ir/paper/248472/en

IEEE: Copy

F. RAKHSHANDEHROO, P. PALUKAITIS, and M. SHAMS BAKHSH, “THE INSTABILITY OF THE INHIBITION OF THE EXPRESSION OF GENES INVOLVED IN RESISTANCE TO VIRUS INFECTION IN TRANSGENIC PLANTS USING HAIRPIN CONSTRUCTS,” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 26, no. 3, pp. 401–415, 2014, [Online]. Available: https://sid.ir/paper/248472/en

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