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Information Journal Paper

Title

EXTRACTION OF A POLYGALACTURONASE FROM MACROPHOMIONA PHASEOLINA AND ANALYSIS OF ITS STABILITY

Pages

  234-243

Abstract

POLYGALACTURONASES (GH28) hydrolyze the pectic substances, present mostly in plants. POLYGALACTURONASES are widely distributed among microorganisms and higher plants. Microbial POLYGALACTURONASES, mainly produced by fungi, are utilized in many largescale industrial processes. Production of a polygalacturonase from a fungal phytopathogen, M. phaseolina was evaluated. M. phaseolina was cultured in production medium. The crude extract was separated from fungal cells. The highest enzyme activity was observed at 60oC. This enzyme had a high stability at wide ranges of pH and temperature and exhibited a t1.2 of 400 min at 70oC. The value for free energy of the heat inactivation at the temperature of 70oC was about 108 kJ, suggesting its potential to be used as an industrial enzyme. Analysis of biochemical characteristics of polygalacturonase from different fungi using PROTPARAM demonstrated that the M. phaseolina polygalacturonase was amongst the most stable fungal POLYGALACTURONASES.

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    APA: Copy

    FAHIMI BAYRAMI, ELNAZ, FARROKHI, NASER, & AMINZADEH, SAEED. (2016). EXTRACTION OF A POLYGALACTURONASE FROM MACROPHOMIONA PHASEOLINA AND ANALYSIS OF ITS STABILITY. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 29(2 ), 234-243. SID. https://sid.ir/paper/248522/en

    Vancouver: Copy

    FAHIMI BAYRAMI ELNAZ, FARROKHI NASER, AMINZADEH SAEED. EXTRACTION OF A POLYGALACTURONASE FROM MACROPHOMIONA PHASEOLINA AND ANALYSIS OF ITS STABILITY. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2016;29(2 ):234-243. Available from: https://sid.ir/paper/248522/en

    IEEE: Copy

    ELNAZ FAHIMI BAYRAMI, NASER FARROKHI, and SAEED AMINZADEH, “EXTRACTION OF A POLYGALACTURONASE FROM MACROPHOMIONA PHASEOLINA AND ANALYSIS OF ITS STABILITY,” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 29, no. 2 , pp. 234–243, 2016, [Online]. Available: https://sid.ir/paper/248522/en

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