STUDY OF DIRECT PLANTLET REGENERATION IN TWO POPULATIONS OF MEDICINAL BITTER MELON (CITRULUS COLOSYNTHIS L.) USING TYPES OF EXPLANTS AND PLANT GROWTH REGULATORS

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GHASEMI Z.; MASOUMIASL A.; AMIRI FAHLIANI R.

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Journal: Journal of Molecular and Cellular Researches Year:2017 | Volume:30 | Issue:3 Page(s): 402-412

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Title

STUDY OF DIRECT PLANTLET REGENERATION IN TWO POPULATIONS OF MEDICINAL BITTER MELON (CITRULUS COLOSYNTHIS L.) USING TYPES OF EXPLANTS AND PLANT GROWTH REGULATORS

Author(s)

GHASEMI Z. | MASOUMIASL A. | AMIRI FAHLIANI R. | Issue Writer Certificate

Keywords

SHOOTINGQ1

ROOTINGQ2

BENZYLAMINOPURINEQ1

GENOTYPEQ2

Abstract

Bitter melon is an anticancer medicinal plant that its saponins and flovonoids induce immunity system of body, thus it is one of the most important medicinal plants. Direct plantlet regeneration from Iranian accessions of this plant for itsin vitro propagation will be very functional. For this, an experiment was conducted in factorial based on Complete Randomized Design. It’s factors included explants (shoot and leaf), PGRs (different levels of BAP ad NAA), culture medium (complete MS, ½MS and¼MS) and population (Gachsaran and Dehdasht). In Dehdasht population, shoot explants were better than leaf explants, but in Gachsaran population was similar for both explants. In respect to shoot regeneration percent, shoot number and shoot length, PGRs combination of 1.5 mg.L-1 BAP together with 1 mg.L-1 NAA in ½MS showed the best direct regeneration in both populations (44.4 and 33.3 percent, respectively). The best medium for ROOTING in shoot obtained from regeneration was MS medium containing 0.5 mg.L-1 BAP and 0.5 mg.L-1 NAA (76.8 and 73.33 percent, respectively). The results of this research showed that these two assessed Iranian populations have good response to in vitro culture and direct plantlet regeneration.

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APA: Copy

GHASEMI, Z., MASOUMIASL, A., & AMIRI FAHLIANI, R.. (2017). STUDY OF DIRECT PLANTLET REGENERATION IN TWO POPULATIONS OF MEDICINAL BITTER MELON (CITRULUS COLOSYNTHIS L.) USING TYPES OF EXPLANTS AND PLANT GROWTH REGULATORS. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 30(3 ), 402-412. SID. https://sid.ir/paper/248536/en

Vancouver: Copy

GHASEMI Z., MASOUMIASL A., AMIRI FAHLIANI R.. STUDY OF DIRECT PLANTLET REGENERATION IN TWO POPULATIONS OF MEDICINAL BITTER MELON (CITRULUS COLOSYNTHIS L.) USING TYPES OF EXPLANTS AND PLANT GROWTH REGULATORS. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2017;30(3 ):402-412. Available from: https://sid.ir/paper/248536/en

IEEE: Copy

Z. GHASEMI, A. MASOUMIASL, and R. AMIRI FAHLIANI, “STUDY OF DIRECT PLANTLET REGENERATION IN TWO POPULATIONS OF MEDICINAL BITTER MELON (CITRULUS COLOSYNTHIS L.) USING TYPES OF EXPLANTS AND PLANT GROWTH REGULATORS,” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 30, no. 3 , pp. 402–412, 2017, [Online]. Available: https://sid.ir/paper/248536/en

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