STUDY OF THE EFFECT OF ROLC AND TROLC GENES ON CHLOROSIS IN TOBACCO PLANTS

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GARDOONPAR H.; MOHAJJEL SHOJA H.; HOSSEINPOUR FEIZI M.A.

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Journal: Journal of Molecular and Cellular Researches Year:2015 | Volume:28 | Issue:4 Page(s): 579-587

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Title

STUDY OF THE EFFECT OF ROLC AND TROLC GENES ON CHLOROSIS IN TOBACCO PLANTS

Author(s)

GARDOONPAR H. | MOHAJJEL SHOJA H. | HOSSEINPOUR FEIZI M.A. | Issue Writer Certificate

Keywords

AGROBACTERIUMQ2

CHLOROSISQ1

RT-PCRQ2

ROLCQ2

TROLCQ2

Abstract

Therol (rooting locus) genes are located on T-DNA (Transferred DNA) of AGROBACTERIUM rhizogenesand induce the morphological and developmental abnormalities upon the integration in to the plant genome. In this study we investigated the effect of bacterialrolC gene and its homologue in Tobacco -tobacco ROLC or 'trolC' gene- on growth of Nicociana tabaccum cv. samsun plants. In the first time, the presence of ROLC and TROLC genes was confirmed in transgenic lines and then using RTPCR technique, we have shown that the transgenic plants expressing the bacterialrolC or tobaccotrolC genes -which demonstrate the chlorotic phenotype-, have very low or no expression of the chloroplastic gene 'psbA'. This gene encodes for one of the proteins of photosystem II. We conclude that the lack of expression of chloroplastic psbAgene and thus the defective feature of chloroplasts in ROLC/trolC transgenic plants may cause the chlorotic phenotype of these plants.

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APA: Copy

GARDOONPAR, H., MOHAJJEL SHOJA, H., & HOSSEINPOUR FEIZI, M.A.. (2015). STUDY OF THE EFFECT OF ROLC AND TROLC GENES ON CHLOROSIS IN TOBACCO PLANTS. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 28(4), 579-587. SID. https://sid.ir/paper/248569/en

Vancouver: Copy

GARDOONPAR H., MOHAJJEL SHOJA H., HOSSEINPOUR FEIZI M.A.. STUDY OF THE EFFECT OF ROLC AND TROLC GENES ON CHLOROSIS IN TOBACCO PLANTS. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2015;28(4):579-587. Available from: https://sid.ir/paper/248569/en

IEEE: Copy

H. GARDOONPAR, H. MOHAJJEL SHOJA, and M.A. HOSSEINPOUR FEIZI, “STUDY OF THE EFFECT OF ROLC AND TROLC GENES ON CHLOROSIS IN TOBACCO PLANTS,” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 28, no. 4, pp. 579–587, 2015, [Online]. Available: https://sid.ir/paper/248569/en

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