CLONING, EXPRESSION AND CHARACTERIZATION OF CHIMERIC BACILLUS THERMOCATENULATUS LIPASE IN E. COLI

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KHALEGHINEJAD S.H.; KARKHANE A.A.; MOTALEB GH.R.; AMINZADEH S.; YAKHCHALI B.

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Journal: Journal of Molecular and Cellular Researches Year:2015 | Volume:28 | Issue:2 Page(s): 202-210

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Title

CLONING, EXPRESSION AND CHARACTERIZATION OF CHIMERIC BACILLUS THERMOCATENULATUS LIPASE IN E. COLI

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Keywords

E. COLIQ2

BTL2Q2

CHIMERIC LIPASEQ2

CLONINGQ2

Abstract

Bacterial lipases are members of a/b hydrolase family that hydrolyzed triacylglycerol at the water- lipid interface. Bacillus thermocatenulatus lipase 2 (BTL2) is a thermoalkalophilic lipase that shows optimal activity at 60–75oC and pH 8–10. BTL2 is an important research target because of its potential industrial applications. At the present study chimeric Bacillus thermocatenulatus lipase contain the consensus sequence of Candida rugosa lipase (207Gly-Glu-Ser-Ala-Gly211) at the nucleophilic elbow region was cloned and expressed in E. COLI as secretion protein. Finally, Catalytic activity of CHIMERIC LIPASEs was evaluated at presence of various triglycerides as substrates and the effects of different parameters such as temperature, pH, detergents, organic solvents and metal ions were evaluated on chimeric enzyme activity using a pHstat assay. The results showed that the chimeric enzyme is most active to C4 substrate, (pH 9.0) and 60oC. As well as enzyme activity has increased in the presence of organic solvents, N-Hexane, N-heptane, methanol, chloroform and detergents such as Triton X -100, Tween 20, Tween 40. Also, metal ions, respectively decreased general effect on the enzyme activity in CHIMERIC LIPASE.

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APA: Copy

KHALEGHINEJAD, S.H., KARKHANE, A.A., MOTALEB, GH.R., AMINZADEH, S., & YAKHCHALI, B.. (2015). CLONING, EXPRESSION AND CHARACTERIZATION OF CHIMERIC BACILLUS THERMOCATENULATUS LIPASE IN E. COLI. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 28(2), 202-210. SID. https://sid.ir/paper/248613/en

Vancouver: Copy

KHALEGHINEJAD S.H., KARKHANE A.A., MOTALEB GH.R., AMINZADEH S., YAKHCHALI B.. CLONING, EXPRESSION AND CHARACTERIZATION OF CHIMERIC BACILLUS THERMOCATENULATUS LIPASE IN E. COLI. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2015;28(2):202-210. Available from: https://sid.ir/paper/248613/en

IEEE: Copy

S.H. KHALEGHINEJAD, A.A. KARKHANE, GH.R. MOTALEB, S. AMINZADEH, and B. YAKHCHALI, “CLONING, EXPRESSION AND CHARACTERIZATION OF CHIMERIC BACILLUS THERMOCATENULATUS LIPASE IN E. COLI,” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 28, no. 2, pp. 202–210, 2015, [Online]. Available: https://sid.ir/paper/248613/en

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