OPTIMIZATION OF LIPASE PRODUCTION BY BACILLUS SP. ISOLATED FROM ACTIVATED SLUDGE

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HOSSEINI F.; RAZAVIPOUR R.

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Journal: JOURNAL OF MICROBIOLOGY KNOWLEDGE Year:2009 | Volume:1 | Issue:3 Page(s): 1-5

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Title

OPTIMIZATION OF LIPASE PRODUCTION BY BACILLUS SP. ISOLATED FROM ACTIVATED SLUDGE

Author(s)

HOSSEINI F. | RAZAVIPOUR R. | Issue Writer Certificate

Keywords

OPTIMIZATIONQ4

PURIFICATIONQ4

PRODUCTIONQ4

Abstract

Lipases hydrolyze triglycerides to fatty acids and glycerol. Its optimum PRODUCTION depends on to accessibility of triglycerides. Thermophilic and alkalophilic bacterial lipases are important for industries application because they can active in high temperature and alkali conditions. In this research bacterial strain isolated from activated sludge of dairy effluents was identified. Bacterial strain was growth in present of fatty acids as a sole carbon source. Then effects of several carbon (olive oil, sunflower and coconut oil) and organic and inorganic nitrogen sources (peptone, yeast extract, urea, asparagin, ammonium nitrate and ammonium sulfate) were estimated on optimum PRODUCTION of lipase. The best activity was obtained in presence of olive oil and peptone and yeast extract in conditions of pH=8.5 and 50 centigrade degree temperature. For PURIFICATION of crude enzyme in culture media was used from ammonium sulfate precipitation method and DEAE-Sepharose anion exchange chromatography. The enzyme was purified about 30-fold after passing all this levels. The molecular mass was found to be 310KDa which resulted in a single peak by 10% SDS-PAGE chromatography. Enzyme activity of final product was 0.58U/ml. This research was shown lipase PRODUCTION by accessible source such as activated sludge and economic PURIFICATION methods. This product would be extremely favorable for industrial use.

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APA: Copy

HOSSEINI, F., & RAZAVIPOUR, R.. (2009). OPTIMIZATION OF LIPASE PRODUCTION BY BACILLUS SP. ISOLATED FROM ACTIVATED SLUDGE. JOURNAL OF MICROBIOLOGY KNOWLEDGE, 1(3), 1-5. SID. https://sid.ir/paper/193225/en

Vancouver: Copy

HOSSEINI F., RAZAVIPOUR R.. OPTIMIZATION OF LIPASE PRODUCTION BY BACILLUS SP. ISOLATED FROM ACTIVATED SLUDGE. JOURNAL OF MICROBIOLOGY KNOWLEDGE[Internet]. 2009;1(3):1-5. Available from: https://sid.ir/paper/193225/en

IEEE: Copy

F. HOSSEINI, and R. RAZAVIPOUR, “OPTIMIZATION OF LIPASE PRODUCTION BY BACILLUS SP. ISOLATED FROM ACTIVATED SLUDGE,” JOURNAL OF MICROBIOLOGY KNOWLEDGE, vol. 1, no. 3, pp. 1–5, 2009, [Online]. Available: https://sid.ir/paper/193225/en

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