PURIFICATION AND DETERMINATION OF KINETICS PARAMETERS OF ASPARAGINASE FROM SERRATIA MARCESCENS

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ABKHOEI L.; MINAEI TEHRANI D.; KOLAHDOOZ MOHAMADI MINA; EFTEKHAR F.; KARAJI N.; MIRFAKHAR F.; VAZIRITABAR N.

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Journal: Journal of Molecular and Cellular Researches Year:2015 | Volume:28 | Issue:2 Page(s): 145-153

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Title

PURIFICATION AND DETERMINATION OF KINETICS PARAMETERS OF ASPARAGINASE FROM SERRATIA MARCESCENS

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Keywords

ENZYMEQ2

ASPARAGINASEQ1

SERRATIA MARCESCENSQ2

PURIFICATIONQ2

KINETIC PARAMETERSQ2

Abstract

Asparaginase is a hydrolytic ENZYME that catalyses asparagine to aspartic acid and ammonia. The ENZYME is used for treatment of acute lymphoblastic leukemia (ALL), lymphoma and melanolymphoma. S. marcescens is a Gram-negative bacterium with red pigment that can produce ASPARAGINASE in culture medium culture in the presence of asparagine. In this study ASPARAGINASE was purified and KINETIC PARAMETERS of ENZYME were determined. A modified culture medium was used for ENZYME production containing malt extract, soy peptone and asparagine. ENZYME activity was detected after 8 hours and maximum ENZYME activity was reached after 40-44 hours of incubation. PURIFICATION of the ENZYME was carried out using ammonium sulfate precipitation. The concentrated ENZYME preparation was loaded onto DEAE cellulose chromatography column. The SDS-PAGE was determined a single band with about 54 kDa protein. The optimum pH and temperature of ENZYME activity were determined to be about 7 and 40oC respectively, and the KINETIC PARAMETERS such as Km and Vmax were measured for the first time in this study.

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APA: Copy

ABKHOEI, L., MINAEI TEHRANI, D., EFTEKHAR, F., KOLAHDOOZ MOHAMADI, MINA, KARAJI, N., MIRFAKHAR, F., & VAZIRITABAR, N.. (2015). PURIFICATION AND DETERMINATION OF KINETICS PARAMETERS OF ASPARAGINASE FROM SERRATIA MARCESCENS. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 28(2), 145-153. SID. https://sid.ir/paper/248615/en

Vancouver: Copy

ABKHOEI L., MINAEI TEHRANI D., EFTEKHAR F., KOLAHDOOZ MOHAMADI MINA, KARAJI N., MIRFAKHAR F., VAZIRITABAR N.. PURIFICATION AND DETERMINATION OF KINETICS PARAMETERS OF ASPARAGINASE FROM SERRATIA MARCESCENS. JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2015;28(2):145-153. Available from: https://sid.ir/paper/248615/en

IEEE: Copy

L. ABKHOEI, D. MINAEI TEHRANI, F. EFTEKHAR, MINA KOLAHDOOZ MOHAMADI, N. KARAJI, F. MIRFAKHAR, and N. VAZIRITABAR, “PURIFICATION AND DETERMINATION OF KINETICS PARAMETERS OF ASPARAGINASE FROM SERRATIA MARCESCENS,” JOURNAL OF MOLECULAR AND CELLULAR RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 28, no. 2, pp. 145–153, 2015, [Online]. Available: https://sid.ir/paper/248615/en

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