THE EFFECTS OF METFORMIN AND BEE VENOM ON IN VITRO MATURATION OF ISOLATED PREANTRAL FOLLICLES IN MICE

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AZARNIA M.; NABUNI M.; KOUCHESFAHANI MOHSENI H.; TOUSU A.

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Journal: Journal of Animal Research Year:2013 | Volume:26 | Issue:3 Page(s): 228-236

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Title

THE EFFECTS OF METFORMIN AND BEE VENOM ON IN VITRO MATURATION OF ISOLATED PREANTRAL FOLLICLES IN MICE

Author(s)

AZARNIA M. | NABUNI M. | KOUCHESFAHANI MOHSENI H. | TOUSU A. | Issue Writer Certificate

Keywords

MOUSE PREANTRAL FOLLICLESQ2

METFORMINQ2

BEE VENOMQ1

IN VITRO MATURATION (IVM)Q2

Abstract

Metformin has multiple benefits in patients with type 2 diabetes. This drug is also used for treatment of patients with Polycystic ovarian syndrome (PCOS), menstruation cycles improvement and ovulation induction. Other studies showed that meformin cause meiotic progression of mice oocytes in in vitro condition via activation of Adenosin monophosphate-activated protein kinase (AMPK). BEE VENOM is a complex compound, consists of enzymes, proteins, peptides and cytokines. Our previous studies have showed the positive effects of BEE VENOM on PCOS and development of ovarian follicles in Wistar rat model. In this study, we focused on the effects of METFORMIN and BEE VENOM both; in development and surviving rate of mouse follicles and their maturation. Ovaries were taken from 14 days old mice. Then mechanically isolated preantral follicles were cultured in α-MEM supplemented with 5% FBS, 100 mIU/ml FSH, 1% insulin-transferrin-selenium under mineral oil for 14 days, and added 10-5 M METFORMIN and optimum dose of 1 mg/ml BEE VENOM (based on dosimetric experiments) in treated groups. At day 12 of culture, final oocyte maturation and ovulation was induced by the addition of 1.5 IU/ml hCG and 20 ng/ml (Recombinant epidermal growth factor) rEGF. The in vitro effects of METFORMIN and BEE VENOM on mouse preantral follicular growth and subsequent oocyte maturation in all groups were assessed. Our findings showed that diameters of preantral follicles at day2 were increased in METFORMIN group (P<0.01), BEE VENOM group (P<0.05) and co-culture group (P<0.05) significantly. Follicular diameters in day4 also increased in METFORMIN group (P<0.001), BEE VENOM group(P<0.05) and co-culture group (P<0.01) significantly. Survival rates in METFORMIN group and co-culture group increased, but this rate decreased in BEE VENOM group, although there were no significant differences among groups. Germinal vesicle (GV) oocytes percentage in METFORMIN group (P<0.001), BEE VENOM group (P<0.01) and co-culture group decreased (P<0.01) significantly. MІ and MІІ oocytes percentage in all treated groups increased but they were not significant. Based of these results, it was shown that follicles treated by METFORMIN and BEE VENOM in vitro, grew more significant than control group. In addition ovulated oocytes treated by METFORMIN and BEE VENOM showed much higher meiotic progression, maturation and preparation for fertilization. Based of these data could conclude, METFORMIN and BEE VENOM have developmental effect on competence and maturation of ovarian follicles. Therefore, these data could be useful to improve our knowledge in infertility and menstruation disorders.

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APA: Copy

AZARNIA, M., NABUNI, M., KOUCHESFAHANI MOHSENI, H., & TOUSU, A.. (2013). THE EFFECTS OF METFORMIN AND BEE VENOM ON IN VITRO MATURATION OF ISOLATED PREANTRAL FOLLICLES IN MICE. JOURNAL OF ANIMAL RESEARCH (IRANIAN JOURNAL OF BIOLOGY), 26(3), 228-236. SID. https://sid.ir/paper/248660/en

Vancouver: Copy

AZARNIA M., NABUNI M., KOUCHESFAHANI MOHSENI H., TOUSU A.. THE EFFECTS OF METFORMIN AND BEE VENOM ON IN VITRO MATURATION OF ISOLATED PREANTRAL FOLLICLES IN MICE. JOURNAL OF ANIMAL RESEARCH (IRANIAN JOURNAL OF BIOLOGY)[Internet]. 2013;26(3):228-236. Available from: https://sid.ir/paper/248660/en

IEEE: Copy

M. AZARNIA, M. NABUNI, H. KOUCHESFAHANI MOHSENI, and A. TOUSU, “THE EFFECTS OF METFORMIN AND BEE VENOM ON IN VITRO MATURATION OF ISOLATED PREANTRAL FOLLICLES IN MICE,” JOURNAL OF ANIMAL RESEARCH (IRANIAN JOURNAL OF BIOLOGY), vol. 26, no. 3, pp. 228–236, 2013, [Online]. Available: https://sid.ir/paper/248660/en

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