Investigating the apoptotic effects of silver nanoparticles coated with Achillea biebersteinii extract on A2780 ovarian cancer cells

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BAHARARA JAVAD; RAMEZANI TAYEBEH; SAGHIRI NEGAR; Salek Farzaneh

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Journal: NOVA BIOLOGICA REPERTA Year:2019 | Volume:6 | Issue:2 Page(s): 140-147

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Title

Investigating the apoptotic effects of silver nanoparticles coated with Achillea biebersteinii extract on A2780 ovarian cancer cells

Author(s)

BAHARARA JAVAD | RAMEZANI TAYEBEH | SAGHIRI NEGAR | Salek Farzaneh | Issue Writer Certificate

Keywords

caspaseQ2

cytotoxicityQ1

green synthesisQ1

medicinal plantQ1

nanoparticlesQ1

Abstract

In recent years using silver nanoparticles (AgNPs) has increased due to its unique properties. The medicinal plant Achillea biebersteinii is rich in anti-cancer compounds. The aim of this study was to investigate the cytotoxicity of AgNPs synthesized using Achillea biebersteinii extract on human ovarian cancer A2780 cells. Cytotoxic effects of AgNPs with MTT test was performed in a 48-hour period in concentrations of 2, 4, 8, 16 and 32 μ g/ml. To study the cell death induced by AgNPs, the staining methods of DAPI and acridine orange (AO)/ Propidium iodide (PI) were executed, Annexin v/Propidium iodide assays were performed and the activation of caspase 3 and 9 was assessed. The results showed that the synthesized AgNPs decreased cell viability in a dose-dependent manner, inhibitory concentration of 50 % (IC50) was calculated as 4 μ g/ml. The results of AO/PI, DAPI staining methods and Annexin V/PI showed that, in treated groups, the percentage of apoptotic cells increased as compared with the control group. In addition, the treated groups showed increased activation of caspase 3 and 9. Therefore, silver nanoparticles induced cell death through the caspase-dependent pathway. The results suggest that AgNPs synthesized by the use of Achillea biebersteinii extract exert their cytotoxic effect on A2780 cells by inducing apoptosis.

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APA: Copy

BAHARARA, JAVAD, RAMEZANI, TAYEBEH, SAGHIRI, NEGAR, & Salek, Farzaneh. (2019). Investigating the apoptotic effects of silver nanoparticles coated with Achillea biebersteinii extract on A2780 ovarian cancer cells. NOVA BIOLOGICA REPERTA, 6(2 ), 140-147. SID. https://sid.ir/paper/250483/en

Vancouver: Copy

BAHARARA JAVAD, RAMEZANI TAYEBEH, SAGHIRI NEGAR, Salek Farzaneh. Investigating the apoptotic effects of silver nanoparticles coated with Achillea biebersteinii extract on A2780 ovarian cancer cells. NOVA BIOLOGICA REPERTA[Internet]. 2019;6(2 ):140-147. Available from: https://sid.ir/paper/250483/en

IEEE: Copy

JAVAD BAHARARA, TAYEBEH RAMEZANI, NEGAR SAGHIRI, and Farzaneh Salek, “Investigating the apoptotic effects of silver nanoparticles coated with Achillea biebersteinii extract on A2780 ovarian cancer cells,” NOVA BIOLOGICA REPERTA, vol. 6, no. 2 , pp. 140–147, 2019, [Online]. Available: https://sid.ir/paper/250483/en

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